Myricetin is a naturally occurring bioflavonoid found in various fruits, vegetables, herbs, and beverages such as tea and wine. It possesses anti-oxidative, anti-inflammatory, and anti-cancer benefits, thus playing a pivotal role in preventive healthcare. To fully understand and harness these pharmacological effects, accurate analysis of myricetin is vital.
Creative Proteomics provides an extensive, precise, and reliable myricetin analysis service. The identification and quantification of myricetin and its derivatives aid in evaluating their therapeutic potential and metabolism in the body. Additionally, this analysis provides important insights into the biochemical, physiological, and pathological roles of myricetin in human health and disease.
Myricetin
Advancements in analytical techniques such as Mass Spectrometry have allowed the wide-ranging study of myricetin. At Creative Proteomics, we employ cutting-edge technologies, like Liquid Chromatography-Mass Spectrometry (LC-MS), and Gas Chromatography-Mass Spectrometry (GC-MS).
Liquid Chromatography-Mass Spectrometry (LC-MS): Ideal for analyzing heat-sensitive and non-volatile samples, LC-MS is extensively used for the detection of myricetin. The use of LC-MS/MS (Tandem Mass Spectrometry) using QTRAP system extends the sensitivity and provides data on structural identity.
Gas Chromatography-Mass Spectrometry (GC-MS): Hailed for its high efficiency and resolution, GC-MS is suitable for volatile samples. Thermo Fisher's Q Exactive GC Orbitrap GC-MS/MS system is available in our labs for delivering accurate myricetin analysis.
Workflow for Plant Metabolomics Service
Myricetin | Myricetin 3-O-glucoside | Myricetin 3-O-galactoside | Myricetin 3-O-rhamnoside |
Myricetin 3-O-arabinoside | Myricetin 3-rutinoside | Myricetin Hexoside | Myricetin Pentoside |
Myricitrin |
Sample Type | Sample Quantity Required |
---|---|
Blood | 1–2 ml |
Urine | 10–100 ml |
Plant tissue | 100–1000 mg |
Food Products | 100–1000 mg |
Our clients receive:
Creative Proteomics is dedicated to offering a comprehensive range of myricetin analysis services using cutting-edge technologies and a team of seasoned analysts. Harness our expertise to fuel your myricetin research endeavors to new heights.
Learn more about other flavonol analysis.
Case. Investigating the Stability of Myricetin in DMEM Using In Situ UPLC–MS–MS Analysis
Background
Flavonols, particularly myricetin, are abundant dietary polyphenols known for their health benefits. However, myricetin's stability during processing and storage is a critical but often overlooked factor. This study aims to address this gap by employing in situ UPLC–MS–MS analysis to investigate the stability of myricetin in Dulbecco's modified Eagle's medium (DMEM) under different temperatures.
Sample
The primary sample investigated in this study is myricetin, a flavonol with a pyrogallol structure. The myricetin standard solution (5 × 10−3 mol/L) was prepared in dimethyl sulfoxide (DMSO). The stability of myricetin was analyzed in DMEM under two temperatures: 37°C and 4°C.
Technical Platform and Procedure
Chemical Preparation:
In Situ UPLC–MS–MS Analysis:
Reaction Kinetics and Arrhenius Analysis:
Characterization of New Products:
Results
Stability in DMEM at 37°C:
Stability in DMEM at 4°C:
Reaction Kinetics:
Characterization of New Products:
MS–MS chromatogram of new products of myricetin in DMEM
Reference