Homoeriodictyol is a flavonoid compound commonly found in various plant species, including Aloe and Scutellaria. It exhibits a range of biological activities, including antioxidant, anti-inflammatory, antimicrobial, and anticancer properties.
The significance of analyzing Homoeriodictyol lies in its potential medicinal value and widespread occurrence in medicinal plants. By quantifying its content, researchers and manufacturers can assess the potency and consistency of herbal products or pharmaceutical formulations containing Homoeriodictyol. Moreover, understanding the levels of Homoeriodictyol in plants can facilitate scientific investigations into its pharmacological mechanisms and its potential applications in health and disease management.
Homoeriodictyol
Creative Proteomics offers specialized homoeriodictyol analysis services tailored to the needs of researchers and product developers. Our state-of-the-art laboratories ensure high-quality, accurate results.
Homoeriodictyol Quantitative and Qualitative Analysis
Homoeriodictyol Metabolic Profiling
Homoeriodictyol Stability and Degradation Studies
Homoeriodictyol Bioavailability and Pharmacokinetics
Homoeriodictyol Regulatory Compliance Support
Custom Analytical Services
High-Performance Liquid Chromatography (HPLC): HPLC is utilized for the precise quantification of homoeriodictyol, with methods optimized for peak resolution and separation efficiency, critical for accurate analysis in complex biological and botanical extracts.
Our labs are equipped with the latest HPLC systems, including the Agilent 1260 and 1290 Infinity series, which are known for their robust performance and reliability. These systems are coupled with UV, DAD (Diode-Array Detection), and fluorescence detectors, allowing for high sensitivity and selectivity in the detection of homoeriodictyol across various sample matrices.
Liquid Chromatography-Mass Spectrometry (LC-MS/MS): LC-MS/MS is used for identification and quantification, providing detailed mass spectrometry data that helps confirm the presence and structure of homoeriodictyol. We utilize the Thermo Fisher Scientific Orbitrap and Triple Quadrupole systems to provide high-resolution accurate mass measurements and superior quantitation capabilities. These instruments are essential for sensitive and specific detection of homoeriodictyol, even at low concentrations.
Nuclear Magnetic Resonance (NMR) Spectroscopy: NMR spectroscopy is employed for structural elucidation and confirmation of homoeriodictyol. This technique allows for the determination of molecular connectivity, stereochemistry, and conformational analysis. By acquiring 1D and 2D NMR spectra, including ^1H, ^13C, COSY, HSQC, and HMBC experiments, we can confidently identify homoeriodictyol and elucidate its chemical structure with high precision.
Data Analysis and Interpretation: Creative Proteomics employs advanced data analysis software and techniques to process and interpret the results obtained from various analytical platforms. Our team of experienced scientists utilizes statistical analysis, multivariate data analysis, and database searching to extract meaningful information from complex datasets, enabling comprehensive characterization and quantification of homoeriodictyol.
Workflow for Plant Metabolomics Service
Sample Type | Sample Volume | Sample Preparation |
---|---|---|
Biological Samples (e.g., plasma, serum, urine) | 100-500 µL | - Centrifuge at 10,000 rpm for 10 minutes to remove debris - Transfer supernatant for analysis |
Botanical Extracts | 10-100 mg | - Dilute with appropriate solvent (e.g., methanol, ethanol) - Filter through a 0.2 µm membrane filter prior to analysis |
Dietary Supplements | 50-200 mg | - Dissolve in suitable solvent (e.g., methanol, acetonitrile) - Centrifuge if necessary to remove insoluble particles |
Pharmaceutical Formulations | As per dosage form | - Extract homoeriodictyol using appropriate extraction method - Filter prior to analysis |
Food Samples | 1-10 g | - Homogenize sample thoroughly - Extract homoeriodictyol using suitable solvent - Centrifuge and filter before analysis |
Case. Development and Validation of HPTLC-Densitometric Method for Simultaneous Quantification of Persicogenin and Homoeriodictyol in Rhus Species
Background
The genus Rhus, commonly known as Sumac, comprises over 250 species with widespread distribution. Various species of Rhus have been traditionally used for medicinal purposes, including treatment of syphilis, gonorrhea, and wound healing. Persicogenin and homoeriodictyol are flavonoid compounds isolated from R. retinorrhoea, exhibiting pharmacological activities such as anticancer and antioxidant properties. However, a validated analytical method for simultaneous quantification of these compounds in Rhus species is lacking.
Sample
Aerial parts of R. retinorrhoea and R. tripartita were collected from the northern region of Saudi Arabia and authenticated by a field taxonomist. Methanol extracts of the samples were used for analysis.
Technical Methods
Sample Preparation: Aerial parts were air-dried, pulverized, and subjected to continuous hot extraction with methanol. The methanol extract was concentrated and vacuum-dried.
Standard Preparation: Homoeriodictyol and persicogenin standards were isolated from R. retinorrhoea and dissolved in methanol. Calibration solutions were prepared in the range of 100–800 ng per band.
HPTLC Analysis: Chromatographic analysis was performed using 20 × 10 cm HPTLC plates. Samples were applied as bands, developed in toluene–ethyl acetate–methanol mobile phase, and quantified at 293 nm using densitometry.
Method Validation: Validation parameters included linearity, precision, accuracy, robustness, LOD, LOQ, and recovery. Precision was evaluated at three QC levels, and accuracy was determined by the standard addition method. Robustness was assessed by introducing small changes in the chromatographic conditions.
Results
The developed HPTLC-densitometric method demonstrated good linearity, precision, accuracy, and robustness. Homoeriodictyol and persicogenin were detected in the methanol extract of R. retinorrhoea but not in R. tripartita. The method provided a precise and selective means for the simultaneous quantification of these compounds in Rhus species, paving the way for further research and quality control applications.
Chromatogram of standards homoeriodictyol (700 ng spot–1; RF =0.30) and persicogenin (700 ng spot–1; RF = 0.48) with mobile phase: toluene–ethyl acetate–methanol (8:2:0.5, v/v).
3D display of all tracks at 293 nm; mobile phase: toluene–ethyl acetate–methanol (8:2:0.5, v/v).
Reference