Catechins are types of flavonoids, categorized under plant secondary metabolites. These are naturally occurring polyphenolic compounds, with potent antioxidant, anti-inflammatory, and antitumor properties. The catechins come in eight stereoisomers depending upon the configurations of two chiral centers present on the molecule. These include (+)-catechin (C), (-)-epicatechin (EC), (+)-gallocatechin (GC), (-)-epigallocatechin (EGC), (-)-catechin-3-gallate (Cg), (-)-gallocatechin-3-gallate (GCg), (-)-epicatechin-3-gallate (ECg), and (-)-epigallocatechin-3-gallate (EGCg).
The powerful antioxidant properties of catechin can significantly reduce oxidative stress by scavenging free radicals, making them highly effective in the prevention and treatment of various chronic diseases, including cancer, diabetes, cardiovascular diseases, and neurodegenerative diseases. Their anti-inflammatory properties offer immense potential in treating inflammation-related conditions. Recognizing their therapeutic potential, an analysis provides crucial insights for their incorporation into functional foods, nutraceuticals, and pharmaceuticals.
Moreover, an accurate catechin analysis forms the basis of quality control for several food and beverage industries, especially those dealing with green tea, dark chocolate, and grape wine. It enables the determination of the grade and quality of the product based on the quantity and proportion of various catechins present.
At Creative Proteomics, we leverage state-of-the-art technologies and world-class expertise to provide accurate, sensitive, and reliable catechin analysis in various sample matrices. This ensures the precise identification and quantification of various Catechins, contributing to improved therapeutic outcomes, product development, and quality control.
Creative Proteomics offers comprehensive catechin analysis services for research, industrial and clinical purposes. We employ a highly trained team of professionals adept in the field of analytic chemistry and bioanalysis, capable of meeting diverse customer requirements whether it is the industry or academia.
Liquid Chromatography-Mass Spectrometry (LC-MS)
Gas Chromatography-Mass Spectrometry (GC-MS)
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Capillary Electrophoresis-Mass Spectrometry (CE-MS)
High-Resolution Mass Spectrometry (HR-MS)
Workflow for Plant Metabolomics Service
Catechin | Epicatechin | Epicatechin gallate | Epigallocatechin | Epigallocatechin gallate |
Gallocatechin | Catechin gallate | Gallocatechin gallate | Epicatechin 3-gallate | Epicatechin 5-gallate |
Epigallocatechin 3-gallate | Epigallocatechin 5-gallate |
Sample Type | Recommended Quantity |
---|---|
Plasma | 200-500 μL |
Serum | 200-500 μL |
Urine | 1-5 mL |
Saliva | 0.5-1 mL |
Feces | 0.5-1 g |
Tissue (e.g., liver) | 50-100 mg |
Hair | 10-20 mg |
Cells (cultured) | 1-5 million cells |
Breath | 5-10 L |
Case. Quantitative Analysis of Catechin and Quercetin in Rosa roxburghii Tratt Using HPLC and HPLC-MS
Background
Rosa roxburghii Tratt, commonly known as silk flower, is a deciduous shrub rich in flavonoid compounds, vitamins, and superoxide dismutase, known for its nutritional and potential therapeutic properties. This study focuses on the quantitative analysis of two key flavonoids, catechin and quercetin, present in Rosa roxburghii Tratt, considering their reported health benefits, including antioxidant effects and potential in cancer treatment.
Sample
The study utilized Rosa roxburghii Tratt samples obtained from a reputable source. The extraction process involved milling the plant into powder, followed by extraction using 70% ethanol at 90°C. Further purification steps, including macroporous resin-packed columns, were employed to isolate flavonoid compounds for analysis.
Technical Platform and Procedure
Standard Curve Construction:
Recovery Test:
Qualitative Analysis:
Contents Determination:
Results
The total flavonoid content, determined using rutin as a standard, exhibited a linear relationship within the tested concentration range, with a content of 73.85%, aligning with national standards.
Recovery tests demonstrated high accuracy, with average recoveries of 98.08% for catechin and 98.50% for quercetin, along with low relative standard deviations, indicating the reliability of the analytical method.
Qualitative analysis confirmed the presence of catechin and quercetin in Rosa roxburghii Tratt samples through comparison of retention times and mass spectra.
The content of catechin and quercetin in the samples was determined using HPLC, and the results were calculated based on the standard curve, providing quantitative insights.
HPLC chromatograms of catechin and quercetin.
Mass spectra of compounds in flavonoid of Rosa roxburghii Tratt (FRT).
Reference