Flavones, a subset of natural compounds belonging to the flavonoids, are widely distributed in various fruits, vegetables, and medicinal plants. Classified as a phenolic class within secondary metabolites, specifically polyphenols, flavones exhibit a distinct double bond between two carbon atoms (C2-C3) in the C ring and comprise two phenolic groups, one of which is a benzene ring.
Due to their pervasive presence across diverse biological species and inherent biological activities, flavones have captured significant attention in the pharmaceutical, food, and health industries. Renowned for their myriad health benefits, including anti-inflammatory, anti-cancer, anti-obesity, and cardiovascular protective effects, flavones stand out as versatile compounds.
Moreover, flavones showcase potent antioxidant properties, positioning them as potential defenders against oxidative stress and related diseases. Their ability to modulate key cellular enzyme function makes them promising therapeutic targets across a spectrum of diseases.
The diverse sub-types of flavones, each characterized by distinct chemical structures and pharmacological effects, emphasize the importance of specific analysis approaches. In this context, conducting a detailed analysis of flavones becomes not only invaluable but also a scientific and industrial imperative.
In simpler terms, flavones analysis plays a crucial role in unraveling their therapeutic potential, ensuring safety, and assessing efficacy. This analysis forms a robust foundation for research and development in pharmaceuticals, nutrition, and cosmetics. At Creative Proteomics, we leverage our expertise and cutting-edge technology to provide a comprehensive flavones analysis service.
Flavones qualitative analysis: Explore the diverse world of flavones compounds in your samples, identifying specific molecules for targeted research.
Flavones quantitative analysis: Accurately measure flavones in various matrices such as food, plasma, and urine, ensuring reliable results.
Flavones structural analysis: Our expert team characterizes complex structures, revealing substitution patterns and conjugate types with precision.
Flavones metabolomics research: We offer a comprehensive analysis of flavones-related metabolic profiling.
Flavones-related microbial metabolism: We will explore the microbial metabolism mechanism of flavones and provide a deeper understanding of the interactions between flavones and organisms.
Personalized flavone analysis: Creative Proteomics can also customize flavones analysis project based on your specific requirements. Our goal is to provide the most accurate and reliable analysis results and to offer a reliable foundation for your research, development or production.
At Creative Proteomics, we employ High-Performance Liquid Chromatography (HPLC) coupled with Tandem Mass Spectrometry (MS/MS) or High Resolution Mass Spectrometry (HRMS) on advanced platforms such as Agilent 1290 Infinity LC system, Thermo Scientific Orbitrap Elite or Thermo Fisher Q Exactive™ series.
By coupling chromatographic separation with mass spectrometry, we achieve reliable detection and quantification of flavones, even in complex biological matrices. The superior sensitivity, selectivity, and accuracy of these instrumentation platforms provide comprehensive profiling and quantification of flavones, enabling their functional studies and quality control.
Workflow for Plant Metabolomics Service
Apigenin | Luteolin | Chrysin | Tangeretin | Baicalein |
Quercetin | Kaempferol | Naringenin | Hesperetin | Rutin |
Morin | Diosmin | Genistein | Fisetin | Nobiletin |
Sample Type | Sample Quantity |
---|---|
Plant Extracts | 50 mg |
Serum | 100 μL |
Cell Culture | 1 x 10^6 cells |
Food Products | 5 g |
Urine | 10 mL |
Tissue Biopsy | 20 mg |
Essential Oils | 1 mL |
Pharmaceutical Formulations | 2 tablets/capsules |
Environmental Samples | 100 mg |
Case. Integration of HPLC and Sensitive NMR for Rapid Identification of Flavonoids
Background:
Flavonoids are a diverse group of plant secondary metabolites with potential health benefits, making their identification crucial in various fields such as herbal medicine, dietary supplements, and food quality control. Traditional methods for flavonoid identification, including HPLC and MS, have limitations, especially in distinguishing isomeric compounds. This study presents an integrated approach using HPLC and NMR for efficient and accurate identification of flavonoids.
Sample:
The study analyzed 46 flavonoids, including flavones, flavonols, and flavanones, covering a range of structures, substitution patterns, and glycosylation states. Plant extracts from elderflowers, heather, lemon peels, and bitter orange peels were used for method validation and applicability.
Technical Platform and Procedure:
Materials and Extraction:
NMR Spectroscopy:
HPLC Analysis:
HPLC-PDA-ESI-MS Analysis:
SPE-Trapping and Elution with Pyridine-d5:
Results
Facile Route for Flavonoid Analysis:
NMR as a Key Tool for Identification:
Database for Rapid Identification:
Application in Plant Extract Analysis:
Advantages and Future Implications:
Comparison of utilized columns in the present study in a k' vs. k'-plot.
HPLC and UHPLC chromatographic profiles of C. vulgaris (a), S. nigra (b) and C. x aurantium (c), C. limon (d), respectively.
Reference