Ubiquitination is the process of covalent binding to a target protein catalyzed by a series of enzymes and is one of the most important regulatory mechanisms for post-translational modification (PTM) of proteins. The study of protein ubiquitination is increasingly playing a crucial role in cell biology and drug discovery. Creative Proteomics has launched an advanced and professional PTM analysis platform. We are able to help our clients perform comprehensive protein ubiquitination analysis and solve research problems in ubiquitination identification and quantification, ubiquitination tandem mass spectrometric (MS/MS) validation, and so on.
The challenge of protein ubiquitination analysis
It is a critical challenge for the characterization of protein ubiquitination, and several reasons are followed:
- Under normal physiological conditions, the stoichiometry of protein ubiquitination is relatively low.
- Ubiquitin can modify substrates with one or more lysine residues at the same time.
- Ubiquitin itself can act as a substrate, resulting in a complex ubiquitin chain with varying lengths, links, and overall architecture.
- Dynamic variability of ubiquitination in vivo.
Protein ubiquitination analysis service
To better understand the function of protein ubiquitination, more approaches or strategies are required to globally paint the picture of protein ubiquitination. Based on our professional platform, we provide detailed and comprehensive services to help clients better solve their research problems. Specifically, our services include experimental design, sample preparation (protein extraction, digestion, peptide, isolation, and enrichment), LC-MS/MS analysis, MS data analysis, and a variety of bioinformatics analyses. Therefore, we can help customers to achieve a large-scale qualitative and quantitative analysis of ubiquitinated proteins.
Our specific services:
In addition, our experts can help clients solve other research problems, such as accurate prediction of ubiquitination sites, ubiquitination MS/MS validation, in vitro ubiquitin conjugation reaction detection, and protein ubiquitination measurement in living cells.
Service features
- Powerful MS analysis.
- Provide detailed reports, including relevant MS parameters, raw data, details of identified ubiquitination sites, MS images, etc.
- Provide a variety of bioinformatics analyses, such as protein ubiquitination identification (ubiquitinated protein and ubiquitination site identification), protein ubiquitination quantification, functional annotation of ubiquitinated proteins, and motif analysis of ubiquitinated peptides.
- One-stop service: Sample processing - Mass spectrometric analysis - Bioinformatics analysis - Project report.
The application areas we serve are as follows, but are not limited to
- Cellular physiological mechanism research.
- Drug target research.
- Disease marker screening.
- Plant stress/resistance research.
Advantages of our service
- Professional evaluation and experimental design.
- Solving problems with professional knowledge and creativity.
- Complex biological data interpretation.
- Rich experience in PTM analysis.
As a professional PTM analysis services provider, Creative Proteomics offers various PTM analysis services to help our customers to accelerate research progress and obtain better results. If you are interested in our services, please contact us for more details.
References
- Stone, Sophia L. "Role of the ubiquitin proteasome system in plant response to abiotic stress." International review of cell and molecular biology 343 (2019): 65-110.
- Dittmar, Gunnar, and Konstanze F. Winklhofer. "Linear ubiquitin chains: cellular functions and strategies for detection and quantification." Frontiers in Chemistry 7 (2020): 915.
- Sun, Mingwei, and Xiaofei Zhang. "Current methodologies in protein ubiquitination characterization: from ubiquitinated protein to ubiquitin chain architecture." Cell & Bioscience 12.1 (2022): 1-17.
Our products and services are for research use only.
