Secretomics Analysis Service

Secretory proteins are proteins that are synthesized inside the cell and then secreted outside the cell to act. A secretory protein is any protein secreted by a cell, either endocrine or exocrine. Secretory proteins include many hormones, enzymes, toxins, and antimicrobial peptides. In eukaryotes, secretory proteins are proteins that are synthesized on the rough endoplasmic reticulum for "export". In prokaryotes, secretory proteins may be synthesized on ribosomes associated with the plasma membrane or exported post-translationally. Secretory proteins (e.g. cytokines, interleukins, growth factors and hormones) have multiple functions and are involved in cell signaling, regulation of cell proliferation, differentiation and apoptosis, development of the organism and other important life processes.

Understanding the characteristics of secreted protein expression in different developmental and growth periods and under different physiological and pathological conditions and in different cell types is important for understanding life processes. The secretory proteome helps to find biomolecules directly related to specific physiological or pathological states, providing a theoretical basis for understanding the process of disease onset and progression at the molecular level, as well as a basis for designing drug screens that act on specific target molecules.

The expression of secreted proteins is inconsistent in different environments, and the study of protein expression should be precise to the cellular or even subcellular level. The abundance of secreted proteins is lower compared to intracellular proteins and media additives. Therefore, the secretomics analysis needs to be performed with the help of high throughput and high sensitivity instruments.

Creative Proteomics is based on optimized sample pre-processing methods for efficient enrichment of secreted proteins. Data acquisition and visualization are achieved through a highly sensitive, high-throughput nano-UPLC -MS/MS technology platform and professional bioinformatics analysis services. We aim to provide you with high quality secretomics data to accelerate your project progress.

Secretomics Analysis Technical Methods

• Sample pre-processing:

Biological mass spectrometry-based secretomics analysis generally requires obtaining secretory proteins from serum-free conditioned media, which are then enriched and analyzed. We can also provide highly selective labeling and efficient enrichment of secreted proteins based on bioorthogonal chemical biology techniques.

• Data acquisition:

nano-UPLC -MS/MS

General workflow of cell secretome investigations (Song et al., 2019).

Application of Secretory Proteomics Services

• Changes in the secretory proteome under different environmental conditions.

Different developmental and growth periods and different physiological and pathological conditions differ in the expression of secretory proteins expressed by different cell types. Analysis of the secretomics in different environments satisfies various basic studies.

• Search for biomarkers or drug targets.

Secreted proteins may be biomarkers or drug targets for certain diseases. For example, during tumorigenesis and development, tumor cells will secrete a large number of proteins. And some secreted proteins have been used as tumor markers for clinical detection and prognosis of tumors.

• Discovery of new antibiotics and vaccine development.

Secreted proteins are usually associated with virulence factors of pathogenic microorganisms and are involved in processes such as bacterial adhesion to host cells, multiplication and invasion in the host, and inhibition of host cell defense mechanisms. Therefore, secreted proteins may be candidates for drug targets. Current secretomics technologies allow large-scale, high-throughput studies of secretory proteins of various parasites, viruses, etc. Secretomics analysis facilitates the study of pathogenesis of microorganisms, vaccine development and drug development.

Reference

1. Song, P., Kwon, Y., et al. (2019). Secretomics to discover regulators in diseases. International journal of molecular sciences, 20(16), 3893.

* For Research Use Only. Not for use in diagnostic procedures.