Extracellular Vesicles Metabolomics Service

Extracellular vesicles (EVs) are rapidly attracting the attention of researchers as mediators of intercellular communication for biomarker discovery, diagnosis and therapy. EVs are involved in a variety of physiological processes and can integrate various biomolecules. The content components of EVs include mRNA and miRNA, as well as proteins, lipids, and metabolites. Metabolites are an important type of molecule carried by EVs.

Metabolites are a class of small molecules containing a variety of substances with molecular weights less than 2 kDa, such as steroid hormones, amino acids, nutrient anabolic intermediates and lipids. Metabolomics is used to study downstream metabolic changes that reflect the cellular phenotype and provide ideas for the resolution of disease pathogenesis and biological mechanisms. In various diseases, EVs provide new perspectives on altered cellular or tissue states, their detection in biological fluids may provide multicomponent diagnostic information, and therapies can be designed based on the substance information transfer capabilities of EVs for relevance.

Creative Proteomics aims to provide high-quality extracellular vesicles metabolomics services for customers in different fields. Our scientists help speed up your project progress based on professional knowledge and high-sensitivity equipment.

We Provide but Are Not Limited To:

Isolation and enrichment of extracellular vesicles

We have a standard process from collection of body fluid samples or in vitro cultured cell samples to sample analysis, with the option/combination of ultra-centrifugation or density gradient centrifugation, affinity purification, nanotechnology, microfluidic analysis and other methods to obtain purer extracellular vesicles.

Targeted metabolomics service

Use standard products as a reference to perform targeted detection and quantification of a specific metabolite or a certain type of metabolite. Calculate the concentration of metabolites through the standard curve to achieve absolute quantification.

Untargeted metabolomics services

Untargeted metabolomics is an orbitrap-based ultra-high resolution mass spectrometry that enables accurate qualitative and quantitative analysis of metabolites in extracellular vesicle samples. High-throughput untargeted metabolomics analysis by HPLC-MS/MS method provides unbiased detection of all small molecule metabolites in extracellular vesicles. The differential metabolites were then screened using statistical analysis, followed by differential analysis using PCA (principal component analysis) and heat map functions, and metabolic pathways of the differential metabolites to identify the relative relationships between metabolites and physiological and pathological changes.

Technical Advantages

• High sensitivity, resolution
• Wide dynamic range
• Large number of substance characterization: efficient metabolite extraction method, upgraded characterization method
• Diversified biological letter analysis

Applications

• Uncovering pathogenesis
• Finding biomarkers for disease diagnosis and prognosis
• Targets and tools for disease treatment
• Disease risk prediction

Creative Proteomics is based on a highly stable, reproducible and highly sensitive separation, characterization, identification and quantitative analysis system to provide you with a reliable, fast and cost-effective one-stop extracellular vesicles metabolomics analysis service. Thanks for your trust. If you have any questions or need other extracellular vesicles analysis related services, please contact us.

* For Research Use Only. Not for use in diagnostic procedures.