Golgi Apparatus and Golgi Protein Extraction and Purification

Creative Proteomics offers Golgi apparatus isolation and Golgi protein purification services for cultured cells, plants, yeast, tissues. Our service can help you obtain purified Golgi apparatus and Golgi proteins for subsequent assays.

The Golgi apparatus, also known as Golgi complex, is composed of a series of flattened, stacked pounces called cisternae. It is a factory where proteins from endoplasmic reticulum are further modified, processed and sorted for transport to final destinations, including lysosomes, the plasma membrane, or secretion. The Golgi apparatus is also the site where glycolipids, sphingomyelin, and polysaccharides are synthesized. Golgi apparatus plays essential roles in cellular activities as apoptosis trigger, mitotic checkpoint, a stress sensor, and so on. The function of Golgi apparatus has impact on normal cells and abnormal cells, such as cancer cells. Some molecules associated with Golgi apparatus have been found fundamental for cancer development, and has potential as a therapeutic targets. To have further investigate basic cell activities, relationship between the Golgi apparatus or Golgi protein and diseases, post-translational modification, protein transportation, etc., it is necessarily first to isolate the Golgi apparatus and Golgi proteins.

Advantages of Our Golgi Apparatus Isolation and Golgi Protein Purification Services:

• Rich experience in Golgi apparatus separation and purification.
• Obtained pure Golgi apparatus with low impurities.
• Golgi apparatus can be isolated and purified from tissues, culture cells, plants, yeast, etc.
• Protein can be extracted from Golgi apparatus without plasma membrane or other membranous structure contamination.
• We will conduct purity verification tests to ensure that the isolated protein reaches the target purity, otherwise there is no charge.

We Provide the Following Services, including but not Limited to:

• Single organelle separation and purification
• Golgi apparatus separation and purification
• Golgi protein extraction

Methods of Golgi Apparatus and Golgi Protein Extraction and Purification:

1. Single organelle – Golgi apparatus separation and purification
The Golgi membranes has a distinctively low density, compared to any other membrane except the trans-Golgi network and some plasma membranes, so the Golgi stack can be easily separated and purified by differential or density gradient centrifugation. To rapidly isolate Golgi membranes from liver tissues, intact Golgi stacks are separated at a relatively low-speed centrifugation after homogenization using dextran, and then purified using a simple sedimentation on a sucrose density barrier. When isolate Golgi, retention of Golgi tubules, component of homogenization medium is one of the key determinants. If dextran is unacceptable for subsequent experiment, the Golgi stacks can be isolated by processing the light mitochondrial fraction by flotation through a discontinuous sucrose gradient. The self-generated gradient system is highly reproducible and facilitates the handling of large number of samples. However, according to different samples, the homogenization medium and procedure have to be modified to isolate intact Golgi stacks. More specialized and optimal homogenization medium, procedures, and following separation and purification protocols will be administrated to meet the requirements of your project and purpose.

2. Isolation and purification of multiple organelles
If you need multiple organelle proteins, then we will use the organelle fractionation method to separate and enrich the organelle proteins you need.

3. Extraction of Golgi proteins
A rapid protocol based on differential centrifugation will be conducted to extract Golgi proteins from your samples. And solutions and protocols will vary depending on your samples and subsequent experiments.

Delivery

Test design, purity analysis report, extracted Golgi and purified protein sample.

Want to learn more about Golgi proteins characterization, protein-protein interaction analysis, post-translational modification of proteins, and other analysis services? We provide one-stop analysis of Golgi proteomics.

References

1. Graham J.M., Isolation of Golgi Membranes from Tissues and Cells by Differential and Density Gradient Centrifugation. Current Protocol in Cell Biology, (2001) 3.9.1-3.9.242.
2. Tang D. and Wang Y., Golgi Isolation. CSH Protocols; doi:10.1101/pdb. prot075911562.
3. Morre J. and Mollenhauer H.H., Isolation of the Golgi Apparatus from Plant Cells. The Journal of Cell Biology, VOLUME 28, 1964.

* For Research Use Only. Not for use in diagnostic procedures.