Membrane Protein Solution for Antibody Development
Online InquiryG-protein-coupled receptors (GPCRs), ion channels, and other membrane proteins have shown important roles in many important cellular processes and in various diseases. Therefore, they have emerged as attractive targets for pharmacological manipulation. Due to their highly conserved structure and common use in biological processes, it is particularly important that therapeutic interventions against these targets are highly tuned and selective, and thus antibodies offer promising therapeutic options.
Fig.1 Select paths for antibodies targeting membrane proteins. (Salazar, Georgina To'A., et al., 2021)
Challenges in antibody discovery to complex membrane proteins
The diversity of the structures of membrane proteins and membrane-bound enzymes creates an abundance of opportunities for interventions that regulate the specific functions of these proteins. Moreover, this abundance also contributes to the promise of therapeutic antibodies against complex membrane proteins. The structures of antibodies can be engineered in many ways to match various targets. When analyzing families of possible drug target proteins, membrane-associated GPCRs dominate, followed by ion channels, transporter proteins, and membrane-bound enzymes. While these membrane proteins are a key group for therapeutic antibody applications, they also face a variety of challenges.
- Membrane protein expressing
Membrane protein proteins are often expressed in low abundance and require significant time and effort to determine the optimal expression system.
- Membrane protein extraction and purification
When using membrane proteins as antigens, it is often necessary to isolate and extract the membrane protein. However, once the protein is removed from the lipid environment, it is usually unstable and the natural conformation of the protein needs to be stabilized.
- The structural and functional diversity of the membrane proteins
In addition, the structural diversity of integral membrane proteins is also challenged, by the fact that the required antibody epitopes may be small and inaccessible.
Membrane protein-based antibody discovery and development at Creative Proteomics
Creative Proteomics is a leading service provider in complex membrane protein research and analysis. Our customized, results-driven approach leverages a range of advanced technologies and expertise to support antibody discovery and development. Our high-quality pure, stable and functional native membrane proteins are compatible with immunizations, phage displays, and synthetic displays and can help customers greatly improve their antibody discovery. In addition, our services can contribute to the discovery of new mechanisms of action and promote a more reliable and in-depth understanding of antibody/antigen recognition. Specifically, our services include the following lists, but are not limited to:
- Membrane protein production
- Membrane protein-molecule interaction analysis
- Membrane protein structure determination
- Membrane protein microarray screening service
- Membrane proteomics service
Our capability support
What are the technologies that support our membrane protein-based antibody development platform? How can we help you discover and develop antibodies based on membrane proteins? What deliverables will you receive upon project completion? If you have any of these questions, contact us and someone from our team will get back to you shortly.
Reference
- Salazar, Georgina To'A., et al. "Antibody Therapies Targeting Complex Membrane Proteins." Engineering 7.11 (2021): 1541-1551.
* For Research Use Only. Not for use in diagnostic procedures.
