Exosome Analysis Services
Online InquiryExosomes are lipid bilayer membrane vesicles released by the fusion of the restricted membrane of multivesicular bodies with the cytoplasmic membrane, with a diameter of about 30-200 nm and a biconcave disc or cup shape under electron microscopy. Exosomes are capable of transporting their endogenous proteins, lipids, nucleic acids, miRNAs and other informative materials, participating in intercellular communication and physiological metabolic processes of the organism, and are considered to be important carriers of intercellular communication, disease diagnosis and prognostic circulating biomarkers. They show a very promising research value in clinical diagnosis and treatment. Exosomes regulate the biological activity of recipient cells through the proteins, nucleic acids and lipids they carry, thus participating in biological processes such as immune response, antigen presentation, cell migration, cell differentiation, and tumor invasion.
Creative Proteomics has been working with exosome research and analysis for years. Our services can help researchers and professionals to optimize the isolation, purification, and identification of exosomes from various samples as well as perform exosome engineering for therapeutic applications. In addition, we have high-resolution mass spectrometry instruments and a professional bioinformatics team to provide you with exosome proteomic, exosome metabolomic and exosome lipidomic analysis services. We aim to provide high-quality exosome-related services to customers in different fields worldwide.
The Exosome Assays We Offer Include but Are Not Limited to:
| Exosome isolation and purification | Sucrose gradient centrifugation |
| Polymer-based exosome enrichment method | |
| Immunomagnetic bead method | |
| Size exclusion chromatography method | |
| Exosome identification | Nanoparticle tracking analysis (NTA) |
| Electron microscopy analysis | |
| Western blot | |
| Exosome marker assay | Isolation and enrichment of exosomal CD9, CD63, CD81, TSG101, HSP70 proteins |
| Exosome surface protein identification and quantitative analysis | |
| Exosome engineering | Exosome labeling and tracking |
| Cargo loading | |
| Engineered exosome production | |
| Cargo loading assessment | |
| Exosomes labeling and tracking | Exosome fluorescent labeling |
| Fluorescence exosome purification | |
| Fluorescent exosome concentration labeling | |
| Exosome multiomics analysis | Exosome proteomics analysis: Exosome protein profile identification Exosome protein composition analysis Exosome protein expression level analysis Exosome protein differential expression analysis |
| Exosome metabolomics analysis: Exosome differential metabolite screening Qualitative and quantitative analysis of target metabolites/metabolic pathways | |
| Exosome lipidomics analysis: Exosome lipid composition and level analysis Differential expression analysis of exosomal lipid molecules Qualitative and quantitative analysis of targeted lipid molecules | |
| Exosomal biogenesis and identification | Our capabilities will provide strong support for the study of exosome biogenesis and its identification. |
| Exosomal cargo and loading mechanism | Our services can greatly help the study of exosomal cargo and loading mechanism, facilitating the diagnostic and therapeutic applications of exosomes. |
| Exosome function research | In vitro analysis of the function of exosomes In vivo analysis of the function of exosomes |
Untargeted multi-omic analysis of colorectal cancer-specific exosomes (Eylem et al., 2020)
Basic Analysis
- Data quality control and molecular identification
- Expression quantitative analysis
- Differential analysis
- Functional annotation and enrichment analysis
Applications of Exosome Analysis
- Research on mechanisms related to the pathogenesis and progression of metabolic and cardiovascular diseases, neurodegenerative diseases, cancer and other diseases
- Studying the effects of exosomes on tissue function
- Study of exosomes and related inflammatory responses
- Research related to exosomes as disease-specific biomarkers
- Research related to the involvement of exosomes as carriers of drugs in disease treatment
Reference
- Eylem, C. C., Yilmaz, M., Derkus, B., Nemutlu, E., et al. (2020). Untargeted multi-omic analysis of colorectal cancer-specific exosomes reveals joint pathways of colorectal cancer in both clinical samples and cell culture. Cancer letters, 469, 186-194.
* For Research Use Only. Not for use in diagnostic procedures.
