Exosome-Based Vaccine Development
Online InquiryImmunization aims to induce an adequate immunogenic response, providing a protective umbrella for the host with minimal adverse effects. This is by far the most successful and cost-effective method of eradicating many diseases. In addition to lipid-based nanoparticles, cell-derived exosomes have emerged as a new platform for vaccine delivery in a variety of delivery systems designed to increase antigen presentation and enhance the immune response. Exosomes can be designed to display specific ligands on their surface to target specific cells and can be loaded with different drugs that can be located on the membrane surface or carried within the exosome. Compared to lipid nanoparticles, these intracellular vesicles exhibit lower immunogenicity and significantly reduced toxicity, but with improved drug encapsulation and greater in vivo biodistribution.
Why choose exosomes for vaccine development
- Exosomes and their composition take part in the immune response and multiple disease signaling pathway
Exosomes have been reported to contain a large number of immune response molecules on their surface and carry biological messengers. Dendritic cells (DCs)-derived exosomes can actively prime T cells, contributing to antigen presentation. Moreover, exosomes have been found to play a key role in the chronic inflammatory process.
- Exosomes in stress response
Exosomes have been found to play important roles in regulating intercellular communication and maintaining cellular and systematic homeostasis. Environmental stressors such as oxidative stress, hypoxia, infection and osmotic stress can lead to cellular stress, which in turn affects the content and function of cell-secreted exosomes. Conversely, circulating exosomes also play a fundamental role in immune homeostasis under stressful conditions. Exosomes can present MHC-I and-II antigens, alerting the immune system to the presence of infectious stress.
- Exosomes in the tumor microenvironment (TME) and immune crosstalk
The TME is an adaptive system that is characterized by dynamic and complex. Because of the role of exosomes in the induction of angiogenesis, stromal cell activation, and tumor growth and metastasis, exosomes have the potential in addressing TME proliferation and TME therapeutic resistance.
- Exosomes in infectious diseases
The increased global population, increased global travel, climate change and other factors, greatly expedite the emergence, evolution, and spread of pathogens. Pathogens can use exosomes for growth control, transmission, and virulence coordination. Most viral exosomes carry viral components that can conceal the presence of the virus from the immune system and viral fragments carried by exosomes can also regulate host immune dynamics. Exosomes also have specific receptors that internalize viral components, suggesting that exosomes are vaccine candidates that induce antiviral immunity. In addition, bacterial exosomes have multiple roles, including producing pathogenicity in the host. They can be recognized by the immune system and promote the living of the parent bacterium.
Exosome-based vaccine development at Creative Proteomics
To further promote the development of exosome-based vaccines, Creative Proteomics offers a range of customized and innovation services and solutions, including but not limited to:
| Exosome analysis services | |
|---|---|
| Exosome isolation and purification | Sucrose gradient centrifugation |
| Polymer-based exosome enrichment method | |
| Immunomagnetic bead method | |
| Size exclusion chromatography method | |
| Exosome identification | Nanoparticle tracking analysis (NTA) |
| Electron microscopy analysis | |
| Western blot | |
| Exosome marker assay | Isolation and enrichment of exosomal CD9, CD63, CD81, TSG101, HSP70 proteins |
| Exosome surface protein identification and quantitative analysis | |
| Exosome engineering | Exosome labeling and tracking |
| Cargo loading | |
| Engineered exosome production | |
| Cargo loading assessment | |
| Exosomes labeling and tracking | Exosome fluorescent labeling |
| Fluorescence exosome purification | |
| Fluorescent exosome concentration labeling | |
| Exosome multiomics analysis | Exosome proteomics analysis: Exosome protein profile identification Exosome protein composition analysis Exosome protein expression level analysis Exosome protein differential expression analysis |
| Exosome metabolomics analysis: Exosome differential metabolite screening Qualitative and quantitative analysis of target metabolites/metabolic pathways | |
| Exosome lipidomics analysis: Exosome lipid composition and level analysis Differential expression analysis of exosomal lipid molecules Qualitative and quantitative analysis of targeted lipid molecules | |
| Exosomal biogenesis and identification | Our capabilities will provide strong support for the study of exosome biogenesis and its identification. |
| Exosomal cargo and loading mechanism | Our services can greatly help the study of exosomal cargo and loading mechanism, facilitating the diagnostic and therapeutic applications of exosomes. |
| Exosome function research | In vitro analysis of the function of exosomes In vivo analysis of the function of exosomes |
We are committed to exerting the potential of exosomes as an important candidate for infectious disease vaccine and cancer vaccine development and hope to meet our customers' specific requirements for projects at the preclinical stages. If you are interested in this area, please feel free to contact us. We look forward to providing services for your next project.
References
- Huda, Md Nurul, and Md Nurunnabi. "Potential application of exosomes in vaccine development and delivery." Pharmaceutical Research (2022): 1-37.
- Shimon, Marina Ben, et al. "The Big Potential of Small Particles: Lipid-Based Nanoparticles and Exosomes in Vaccination." Vaccines 10.7 (2022): 1119.
* For Research Use Only. Not for use in diagnostic procedures.
