Mitochondrial Lipidomic Analysis

Lipids are not only components of cell membranes and energy storage substances, but also perform a variety of important biological functions in life activities. Since the analysis of total cells does not represent the specific changes that occur at the organelle level, it is important to determine the lipid composition of each organelle. Lipidomics is the systematic analysis of lipids and molecules that interact with lipids in biological samples. To help our customers gain insight into the mitochondrial lipidome, we offer mitochondrial lipidomic analysis.

Mitochondrial Lipidome

Mitochondria are essential organelles in many processes such as intermediate metabolism, energy harvesting, immune response, and autophagy. Recently, mitochondrial research ( including their number, morphology, and functions) has become the focus of aging and age-related diseases. Mitochondrial function depends on the lipid environment for mitochondrial fission fusion, oxidative phosphorylation and mitochondrial bioenergetics. Moreover, the recruitment and activity of several mitochondrial proteins are also dependent on the lipid environment. Mitochondrial lipid homeostasis is closely related to mitochondrial function, yet little is known about the composition and role of mitochondrial lipids.

Mitochondrial lipidomic analysis at Creative Proteomics

The development of rapid, high-throughput and sensitivity analytical methods, especially the application of soft ionization and high-resolution mass spectrometry (MS), has greatly contributed to the development of lipidomics in the subcellular field. The mitochondrial lipidomic analysis will contribute to the study of mitochondrial function and related diseases through its outstanding ability to directly identify potential functional changes in lipid pathways and signaling networks at the subcellular level. Based on highly stable, reproducible and sensitive systems for separation, characterization, identification and quantification, combined with LC-MS/MS, Creative Proteomics offers reliable, fast, cost-effective and one-stop mitochondrial lipidomics services. We can handle a variety of routine biological specimens, including cells, tissues, and biological fluids. Specifically, our services can help our clients with the following projects, but not limited to:

• Analysis of mitochondrial membrane lipid composition and levels
• Analysis of the differential expression of mitochondrial lipid molecules
• Qualitative and quantitative analysis of mitochondrial lipid molecules

Detection and analysis platform

• Multiple high-resolution mass spectrometers
• High-performance liquid chromatography
• Gas chromatography
• Nuclear magnetic resonance (NMR)

Workflow of our service

• Mitochondrial isolation
• Characterization and verification of mitochondrial samples, including western blot (WB) and transmission electron microscopy (TEM) analysis
• Lipid extraction
• MS-based lipid detection
• Data analysis

We focus on lipid classes mainly found in mitochondria, including phosphatidylcholine (PC), phosphatidylethanolamine (PE), and cardiolipin. In addition to these major lipids, it is clear that there are other lipids that are also important for understanding potential changes in mitochondrial function. Further studies are needed to address and analyze these more minute mitochondrial lipid species. Are you interested in a mitochondrial lipidomic analysis service or would you like to collaborate with us? Please send us a message. Our experts in charge of your area of interest will contact you as soon as possible.

Reference

1. Gaudioso, Angel, et al. "Lipidomic alterations in the mitochondria of aged Parkin null mice relevant to autophagy." Frontiers in Neuroscience 13 (2019): 329.

* For Research Use Only. Not for use in diagnostic procedures.