Mitochondrial Toxicity Detection

Mitochondria play a key role in regulating various cellular physiological mechanisms such as ATP production, metabolic control, immune response, signal transduction and apoptosis. Unfortunately, a variety of drugs and other chemicals can induce mitochondrial toxicity. Agricultural chemicals, industrial chemicals, and pharmaceuticals are particularly known for causing mitochondrial toxicity, doing significant harm to human and environmental health. Sensitive, accurate and specific assays for mitochondrial toxicity and the timely detection of mitochondrial toxicity of compounds early in drug development can help reduce the attrition rate of subsequent clinical trials and save significant development time and costs.

Mitochondrial toxicity detection service

Creative Proteomics offers a reliable, cost-effective, and one-stop solution for mitochondrial toxicity detection based on advanced experimental design and analysis tools. We offer several comprehensive mitochondrial toxicity assessment protocols for evaluating drug-induced mitochondrial toxic effects early in drug discovery. Our services are flexible, cost-effective and high throughput. We excel at handling challenging projects, and our scientists have a wide range of expertise and experience.

• Mitochondrial oxygen consumption detection

In many applicable preclinical organelle models and in vitro cell models, oxygen consumption-based assays are considered being the most informative and specific of the assays used to assess mitochondrial dysfunction and toxicity. Direct assessment of oxygen consumption using the Seahorse XF technique can be a sensitive and specific marker/indicator of mitochondrial toxicity. Our services are available for the detection and quantitative assessment of compounds that trigger mitochondrial toxicity.

• Mitochondrial membrane potential analysis

Observation of the mitochondrial membrane potential can provide information about the basic functions of mitochondria. A decrease in mitochondrial membrane potential is likely to predict damage to mitochondrial structure and function. We are committed to providing the most sensitive and effective assay available for monitoring changes in mitochondrial membrane potential.

• Mitochondrial biogenesis assay

Measurement of mitochondrial biogenesis is a standard part of the early safety assessment of new drugs. Our mitochondrial biogenesis assay simultaneously measures levels of two mitochondrial proteins, COX-1 and SDH-A, and is an ideal way to detect the side effects of the tested compounds on mitochondrial DNA replication and protein synthesis.

• Further research

-Functional mitochondrial toxicity assay

-Mitochondrial Glu/Gal assay

-HCS mitochondrial toxicity

-Mitochondrial oxidative stress

-If mitochondrial toxicity is confirmed during in vitro screening, the mechanism of action can be further investigated with in vitro study assay kits or animal models.

Why choose us?

• Mitochondrial toxicity assessment in drug development
• Standardized mitochondrial toxicity studies
• Parametric assessment of mitochondrial toxicity
• Comprehensive analysis and interpretation of mitochondrial toxicity assay data

Thank you for your interest in our services. We are equipped with state-of-the-art laboratories to provide the widest range of technical support for mitochondrial toxicity detection. Would you like more information about our cellular mitochondrial toxicity assays? Please contact us and our team of experts will get back to you within one business day to discuss your needs.

References

1. Guo, Chunyan, et al. "Oxidative stress, mitochondrial damage and neurodegenerative diseases." Neural regeneration research 8.21 (2013): 2003.
2. Abate, Marianna, et al. "Mitochondria as playmakers of apoptosis, autophagy and senescence." Seminars in cell & developmental biology. Vol. 98. Academic Press, 2020.

* For Research Use Only. Not for use in diagnostic procedures.