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Data-Independent Acquisition (DIA) Proteomics

Accuracy: High-resolution mass spectrometry for precise results.
Stability: 1 ppm accuracy over time, minimal corrections.
Sensitivity: Detects low-abundance proteins (amol levels).
Efficiency: Monitors all proteins at once, faster analysis.
Speed: Rapid 12-20 Hz scanning.
Range: Wide dynamic range for comprehensive quantification.
Selectivity: Enhanced with msxDIA technology.

Reliable Identification: dia-PASEF enhances sensitivity and selectivity.
Speedier Analysis: TIMS separation accelerates while maintaining accuracy.
Comprehensive Coverage: Deeper insights with extensive proteome coverage.
High-Sensitivity Targeting: prm-PASEF boosts target monitoring without compromise.

Tissue Biomarker Discovery Solutions

Discover Your Tissue Biomarkers

With the gradual change of medical treatment from traditional passive treatment to active prevention and diagnosis, especially the emergence and mainstreaming of individualized medicine, the research and development of biomarkers have become a hot topic in the clinical and pharmaceutical fields in recent years. The detection of biomarkers can provide objective information on physiopathological processes, disease development, treatment, and prognosis in normal and disease states. Correspondingly, biomarkers can be classified into different categories according to their various functions. Early detection markers are mainly used for the detection of some early clinical diseases.

The use of DIA (Data-independent acquisition) holographic scanning proteomics technology enables high-throughput detection of large cohorts while ensuring high reproducibility and stability of the technology and data. Among the selected markers, clinical data and machine learning methods are further combined to comprehensively evaluate the markers to verify the reliability of the markers again.

Tissue Proteomics

Routine proteomic analysis of patient tissues has become an important tool for biomarker discovery. Tissue-based proteomic strategies have been applied to the study of many diseases such as cancer. The general idea is to compare disease tissue samples with "healthy" adjacent controls from the same patient in order to find potential diagnostic biomarkers. At the same time, information on the prognosis of patients at different stages of the disease is compared. Once a small number of candidate proteins have been identified, pathway analysis provides insight into how these proteins are associated with disease onset, proliferation, and metastasis. Subsequent antibody-based techniques are often used to complement/validate differential expression results in larger independent cohorts.

Our Tissue Biomarker Discovery Services

Creative Proteomics's tissue biomarker discovery applies DIA MS technologies to overcome the limitations of targeted approaches to identify novel biomarkers. Our advanced technologies in these next-generation proteomics enable the quantification of the complete proteome and phosphoproteome, uncovering new potential biomarkers to accelerate disease diagnostic, treatment, and prognosis diagnostic processes.

The service has unprecedented depth and throughput to identify differentially regulated proteins in a hypothesis-free manner. We also provide highly multiplexed targeted proteomics with absolute quantification capabilities for custom protein panels.

Workflow

Advantages

High accuracy: QC CV as low as 25% for large projects
High-throughput: quantify up to 9,000 proteins per sample
Large-scale: Allow analysis of thousands of samples
Standard data and advanced data analysis

Sample Requirements

Our Tissue biomarker discovery services can be applied to all types of solid biological matrices such as frozen tissue, FFPE slides, or almost any species of cell culture.

Sample Type	Fresh frozen	# of Cells	FFPE slices
Quantify	1 mg	1×10⁷ cells	2×5μm FFPE slices (each 50-100mm²)

Report

Experimental steps
Relevant experiment parameters
Mass spectrometry spectra
Raw data
Proteomics analysis results

Our Tissue Biomarker Discovery Services is ideal for long-term projects or projects with large sample sets that require accurate and reproducible quantitation. Please get in touch with us for more details.

* For Research Use Only. Not for use in the treatment or diagnosis of disease.

ICON 4D Proteomics with Data-Independent Acquisition (DIA)

50% detection rate of low-peak intensity proteins
Higher integrity and reproducibility of protein data
Nearly 100% ion utilization, greatly improving detection sensitivity
Fewer injections, shorter gradients, and deeper coverage
Stronger support for PTM identification

Specializing in proteomics, Creative Proteomics offers cutting-edge protein analysis services. Our distinctive approach revolves around harnessing the power of DIA technology, enabling us to deliver precise and comprehensive insights that drive advancements in research and industry.

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