Glycosylation is the process of transferring glycan to proteins under the action of glycosyltransferases to form glycosidic bonds with amino acid residues. Glycosylation is an important protein modification and plays a crucial role in the proper folding, functional localization, and intracellular transport of proteins.
Among all glycosylation modifications, N-glycosylation is one of the most widely studied cases. N-glycosylation involves in the essential role of sugar chains in various life activities, including cell recognition, cell differentiation, signal transduction, and immune response by linking with the free asparagine's NH2 group of proteins. Moreover, N-glycosylation is significant in the research of embryonic development, cancer development, and immune defense and defense against pathogenic bacteria invasion.
Creative Proteomics uses the novel generation of 4D platform to provide qualitative and quantitative analysis for protein glycosylation and modification sites, which significantly improves sensitivity and depth of coverage. The mass spectrometry technique and enrichment processing allow for large throughput detection of glycosylation-modified proteins with low levels and a wide dynamic range. We provide "one-stop" proteomics services, from large-scale PTMs proteomics screening, PRM validation of targeted proteomics/PTMs omics, to high-quality antibody-assisted validation and subsequent molecular mechanism studies.
Standard Data Analysis | |
Analysis of total protein identification quantity | Protein identification and quantitative result statistics, Venn diagram analysis, number of different results statistics, volcano map, cluster heat map |
Protein function analysis | Subcellular localization, domain analysis, motif analysis, GO function analysis, KEGG pathway analysis, interaction network analysis |
Quality control analysis | Peptide relative molecular mass distribution, peptide sequence length distribution |
Cell: 5×107-108
Protein: >800 µg
Cell collection (try to avoid trypsin); phosphatase inhibitors are required throughout sample processing; protein samples are transported on dry ice.
THERMO Q-EXACTIVE HF,BRUKER TIMS TOF PRO
References:
4D Proteomics with Data-Independent Acquisition (DIA)
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×Specializing in proteomics, Creative Proteomics offers cutting-edge protein analysis services. Our distinctive approach revolves around harnessing the power of DIA technology, enabling us to deliver precise and comprehensive insights that drive advancements in research and industry.