4D Proteomics and Dual-Platform Full-Spectrum Metabolomics

Proteins are the executors of life activities and can directly reflect the protein information of the organism's last function in the face of environmental changes. The metabolome is the biological dynamic regulation system in the stage closest to the phenotype, which is the essential feature and material basis of life. Therefore, when changes in metabolite content are found, it is important to find whether the expression of enzymes upstream of the metabolic pathway Therefore, when changes in metabolite content are found, it is easier to find out whether the expression of the upstream enzymes of the metabolic pathway changes accordingly. On the other hand, it is also easier to find out the reasons for the changes in metabolites and explain the molecular mechanisms of growth, development, physiological status, and pathological responses of organisms.

By integrating and analyzing the histological data at the molecular level of both proteomics and metabolomics, on the one hand, we can combine the analysis results of the two histologies for mutual verification and improve the success rate of subsequent experimental verification. However, they can also work in concert to strengthen one another and improve the systematic nature of the research. Eventually, we will be able to identify the proteins or metabolites associated with important metabolic pathways, develop a model of the molecular biology change mechanism, and obtain a thorough understanding of the overall pattern and direction of biological changes. This will serve as a basis for more in-depth research and analysis.

The new technology, which combines the dual platforms of GC-MS and LC-MS with 4D-DIA proteomics, can better improve the accuracy and comprehensiveness of data from multiple dimensions. It can also mine an increasing amount of accurate data, yielding more valuable results for further in-depth research and application.

Our Research Strategy

• We can increase the success rate of subsequent experimental verification by merging the results of the two histologies for mutual verification and by integrating and evaluating the proteome and metabolomic data at the molecular level.
• By utilizing the proteome and metabolome, the benefits of each complement one another and help to further organize the research. In the end, we are able to attain a thorough comprehension of the overall pattern and course of biological alterations, and we may suggest a molecular biology change mechanism model.
• A database for further in-depth research and analysis will be created by filtering out the proteins or metabolites associated with important metabolic pathways.

Advantages of 4D Proteomics and Dual-Platform Full-Spectrum Metabolomics

Comprehensive analysis. When paired with the dual platform of GC-MS and LC-MS, 4D-DIA proteomic technology can offer thorough analysis at the protein and metabolite levels. This makes it possible for scientists to gather data on metabolites and proteins, which advances our knowledge of the molecular processes that underlie living things.

• High sensitivity. The ability of the GC-MS and LC-MS technologies to analyze chemicals in a sample with high sensitivity and to identify proteins and metabolites at low concentrations is crucial for research involving the detection of low-abundance biomolecules.
• High Resolution. This combination lowers the possibility of confusion and misidentification by providing high-resolution data that aids in the differentiation and identification of various chemicals and proteins.
• Temporal resolution. The examination of temporal resolution made possible by 4D-DIA technology helps research biological processes that are dynamically changing, such as alterations in biological samples throughout time.
• Multi-omics integration. Proteomics and metabolomics data combined enable multi-omics integration, elucidating relationships between proteins and metabolites and advancing our knowledge of biological processes as a whole.

Applications of 4D Proteomics and Dual-Platform Full-Spectrum Metabolomics

• Discovery of Biomarkers. Utilizing these technologies together can aid in the identification of certain molecular markers linked to biological processes, therapeutic responses, or disease.
• Systems Biology Research. Systems biology research can be carried out to gain a deeper understanding of the operation and regulatory processes of biological systems by combining data from proteomics and metabolomics.
• Toxicity Studies. To help find harmful metabolites and comprehend toxicity mechanisms, toxicity studies can make use of this mix of tools.
• Drug Development and Research. This combination can be applied to drug metabolism and protein target studies in the field of drug development, which will aid in the screening and improvement of therapeutic candidates.