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PRM Quantitative Proteomics Services

Parallel Reaction Monitoring (PRM), an alternative method to SRM/MRM, is a targeted quantification method performed on hybrid quadrupole-Orbitrap (q-OT) and quadrupole time-of-flight (q-TOF) mass spectrometers. PRM has comparable sensitivity with similar linearity, precision, repeatability and dynamic range for protein quantification compared with SRM, but PRM provides high specificity, high selectivity, high accuracy and high-throughput quantification with confident targeted protein/peptide confirmation. It is ideal for quantifying tens to hundreds of targeted proteins/peptides in complex matrices.

PRM technology uses the quadrupole of Orbitrap or Q Exactive mass spectrometer to separate the target precursor ion before fragmenting the targeted precursor ion in the collision cell. The product ions can then be detected in the Orbitrap mass analyzer. This allows for accurate specificity analysis of the target proteins/peptides in complex samples.

Analytical Platform

AB SCIEX Triple TOF 5600,AB SCIEX Triple Quad™ 5500, Q-Exactive, Orbitrap Fusion

Workflow

Parallel Reaction Monitoring Workflow

Bioinformatics Analysis

Problems to be SolvedBioinformatics Analysis
Quality Assessment of ProteinPRM Data Analysis
Protein Comparison of Different SamplesMultivariate PCA Analysis
Protein Statistical AnalysisVenn Diagram
Volcano Plot
Functional AnnotationKEGG Annotation
GO Annotation
COG Annotation
Clustering AnalysisHierarchical Clustering
K-Means Clustering
Network AnalysisSTRING Analysis

Sample Requirement

Based on our special protein extraction technology, we can quickly extract proteins from various samples and formulate unique experimental schemes according to different research purposes. Specific requirements are as follows:

Sample TypeProteinCellAnimal TissuePlant TissueBloodUrineSerumMicrobes
Quantify100 ug1×107 cells1 g200 mg1 mL2 mL0.2-0.5 mLDry weighed: 200 mg

Note:

Key Features

Applications

Reports

* For Research Use Only. Not for use in the treatment or diagnosis of disease.

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Specializing in proteomics, Creative Proteomics offers cutting-edge protein analysis services. Our distinctive approach revolves around harnessing the power of DIA technology, enabling us to deliver precise and comprehensive insights that drive advancements in research and industry.

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