Parallel Reaction Monitoring (PRM), an alternative method to SRM/MRM, is a targeted quantification method performed on hybrid quadrupole-Orbitrap (q-OT) and quadrupole time-of-flight (q-TOF) mass spectrometers. PRM has comparable sensitivity with similar linearity, precision, repeatability and dynamic range for protein quantification compared with SRM, but PRM provides high specificity, high selectivity, high accuracy and high-throughput quantification with confident targeted protein/peptide confirmation. It is ideal for quantifying tens to hundreds of targeted proteins/peptides in complex matrices.
PRM technology uses the quadrupole of Orbitrap or Q Exactive mass spectrometer to separate the target precursor ion before fragmenting the targeted precursor ion in the collision cell. The product ions can then be detected in the Orbitrap mass analyzer. This allows for accurate specificity analysis of the target proteins/peptides in complex samples.
Analytical Platform
AB SCIEX Triple TOF 5600,AB SCIEX Triple Quad™ 5500, Q-Exactive, Orbitrap Fusion
Workflow
Bioinformatics Analysis
Problems to be Solved | Bioinformatics Analysis |
Quality Assessment of Protein | PRM Data Analysis |
Protein Comparison of Different Samples | Multivariate PCA Analysis |
Protein Statistical Analysis | Venn Diagram |
Volcano Plot | |
Functional Annotation | KEGG Annotation |
GO Annotation | |
COG Annotation | |
Clustering Analysis | Hierarchical Clustering |
K-Means Clustering | |
Network Analysis | STRING Analysis |
Sample Requirement
Based on our special protein extraction technology, we can quickly extract proteins from various samples and formulate unique experimental schemes according to different research purposes. Specific requirements are as follows:
Sample Type | Protein | Cell | Animal Tissue | Plant Tissue | Blood | Urine | Serum | Microbes |
Quantify | 100 ug | 1×107 cells | 1 g | 200 mg | 1 mL | 2 mL | 0.2-0.5 mL | Dry weighed: 200 mg |
Note:
Key Features
Applications
Reports
4D Proteomics with Data-Independent Acquisition (DIA)
Online Inquiry
×Specializing in proteomics, Creative Proteomics offers cutting-edge protein analysis services. Our distinctive approach revolves around harnessing the power of DIA technology, enabling us to deliver precise and comprehensive insights that drive advancements in research and industry.