4D-DIA proteomics is a next-generation DIA technology based on the timsTOFPro mass spectrometer for differential quantitative proteomics analysis. 4D-DIA proteomics scanning is faster and more sensitive compared to 3D-DIA technology. Meanwhile, the addition of the fourth-dimensional ion drip separation allows DIA data to be acquired without sacrificing window cycling speed while reducing spectral complexity and improving ion utilization, achieving a comprehensive improvement in proteomics in terms of coverage depth, sensitivity, and throughput.
Proteomics techniques can be divided into data-dependent acquisition (DDA) strategies and data-independent acquisition (DIA) strategies.
Using timsTOF Pro, we have launched 4D-DIA proteomics services to help research in microsample proteomics, large cohort of clinical samples, and high-throughput protein analysis.
Standard data analysis content | |
Statistical analysis of identification results | Sample protein and peptide identification histogram, PCA distribution map, quantitative variance statistical analysis |
Bioinformatics analysis | Differential up- and down-regulated protein KEGG map, KEGG pathway function attribution, etc. |
GO and G0 enrichment, KEGG and KEGG enrichment analysis, PPI interaction network and module analysis | |
Advanced data analysis content | |
Biomarker screening | Integrated machine learning, LASSO regression analysis, marker panels, ROC analysis |
Molecular typing analysis | Unsupervised cluster typing analysis, proteome + transcriptome analysis, kinase analysis |
Joint analysis of clinical characterization and omics results | |
Disease-related functional module analysis | WGCNA co-expression analysis |
Survival curve analysis | Clinical typing and survival curve analysis |
Tissue Samples | Protein | # of Cells | Animal Tissue | Plant Tissue | Blood | Urine | Serum | Microbes |
Quantify | 100 ug | 1×107 cells | >200 mg | 1 g | 1 mL | 2 mL | 0.2-0.5 mL | Dry weighed: 200 mg |
In addition, we also provide 4D Label-Free Proteomics and 4D-PTMs Proteomics services. Please contact us for more details.
4D Proteomics with Data-Independent Acquisition (DIA)
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×Specializing in proteomics, Creative Proteomics offers cutting-edge protein analysis services. Our distinctive approach revolves around harnessing the power of DIA technology, enabling us to deliver precise and comprehensive insights that drive advancements in research and industry.