Many studies, such as pathology and drug research, pay more attention to the functions of certain target proteins. Relying on SRM/MRM, PRM and 4D Proteomics technologies, our Targeted Proteomics strategy provides more specific and sensitive multiplexed quantification of target protein/peptides analysis services in complex biological samples. Targeted quantitative proteomic research is increasingly used in diagnostic and pharmaceutical applications to quantify proteins/peptides and metabolites in complex samples. In addition, targeted proteomics are often behind discovery proteomics to quantify specific proteins/peptides found during discovery screening.
Our Targeted Proteomics Services include SRM/MRM and PRM techniques, typically designed to quantify up to 150 proteins with high precision and throughput in a dynamic range of 6 orders of magnitude.
The mass spectrometry of TPro™ is carried out in three stages: isolation of targeted peptides, collision and fragmentation of precursor peptides, and detection of fragmented ions using MS/MS spectrometry. The difference is that MRM is primarily performed on a triple-quadrupole (QQQ) mass spectrometry while PRM is primarily performed on hybrid quadrupole-linear ion trap (QTap) mass spectrometry. Finally, the acquired data is analyzed with the database built by OpenSWATH and Skyline software.
AB SCIEX TripleTOF 5600, AB SCIEX Triple Quad™ 5500, Q Exactive, Orbitrap Fusion
Problems to be Solved | Bioinformatics Analysis |
Quality Assessment of Protein | MRM/PRM Data Analysis |
Protein Comparison of Different Samples | Multivariate PCA Analysis |
Protein Statistical Analysis | Venn Diagram |
Volcano Plot | |
Functional Annotation | KEGG Annotation |
GO Annotation | |
COG Annotation | |
Clustering Analysis | Hierarchical Clustering |
K-Means Clustering | |
Network Analysis | STRING Analysis |
Based on our special protein extraction technology, we can quickly extract proteins from various samples and design unique experiments according to different research purposes. Specific requirements are as follows:
Sample Type | Protein | Cell | Animal Tissue | Plant Tissue | Blood | Urine | Serum | Microbes |
Quantify | 100 ug | 1×107 cells | 1 g | 200 mg | 1 mL | 2 mL | 0.2-0.5 mL | Dry weighed: 200 mg |
Note:
1) Absolute quantitation of multiple proteins/peptides proteins/peptides
2) Biomarker discovery and validation
3) Protein post-translational modification research
4) Target proteins were verification follows quantitative proteomics (such as label-free, iTRAQ)
5) Research of highly homologous protein family members
6) Microorganisms proteomic research
7) Crop proteomics research
4D Proteomics with Data-Independent Acquisition (DIA)
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×Specializing in proteomics, Creative Proteomics offers cutting-edge protein analysis services. Our distinctive approach revolves around harnessing the power of DIA technology, enabling us to deliver precise and comprehensive insights that drive advancements in research and industry.