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SRM-MRM Quantitative Proteomics Services

Selected Reaction Monitoring (SRM), also known as Multiple Reaction Monitoring (MRM), is used to monitor several selected precursor-fragment transitions of the targeted peptides. The selection of the SRM/MRM transitions is calculated based on the acquired data. SRM/MRM, using a triple quadrupole MS, consists of three steps: selection of targeted precursor peptides based on MS spectra, fragmentation one (or more) of these precursor peptides, and acquisition fragment ions based on MS/MS spectra. Up to 6000 transitions can be monitored by SRM/MRM technology. The advantages of SRM/MRM include flexibility, sensitivity, specificity and reproducibility, making it ideal for quantitative analysis which enables the precise quantification of protein networks in biological systems. Furthermore, SRM/MRM based quantification can be combined with different strategies for relative or absolute protein quantification.

Workflow

Selected/Multiple Reaction Monitoring Workflow

Analytical Platform

AB SCIEX Triple TOF 5600,AB SCIEX Triple Quad™ 5500, Q-Exactive, Orbitrap Fusion

Bioinformatics Analysis

Problems to be SolvedBioinformatics Analysis
Quality Assessment of ProteinMRM Data Analysis
Protein Comparison of Different SamplesMultivariate PCA Analysis
Protein Statistical AnalysisVenn Diagram
Volcano Plot
Functional AnnotationKEGG Annotation
GO Annotation
COG Annotation
Clustering AnalysisHierarchical Clustering
K-Means Clustering
Network AnalysisSTRING Analysis

Sample Requirement

Based on our special protein extraction technology, we can quickly extract proteins from various samples and formulate unique experimental schemes according to different research purposes. Specific requirements are as follows:

Sample TypeProteinCellAnimal TissuePlant TissueBloodUrineSerumMicrobes
Quantify100 ug1×107 cells1 g200 mg1 mL2 mL0.2-0.5 mLDry weighed: 200 mg

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Key Features

Applications

Reports

* For Research Use Only. Not for use in the treatment or diagnosis of disease.

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Specializing in proteomics, Creative Proteomics offers cutting-edge protein analysis services. Our distinctive approach revolves around harnessing the power of DIA technology, enabling us to deliver precise and comprehensive insights that drive advancements in research and industry.

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