Protein purification is a complex process, including multiple steps such as coarse separation, preliminary purification and fine purification. Experimental methods include extraction, precipitation, centrifugation, chromatography and other methods.
We have experienced protein purification technicians who can design various protein purification experimental programs such as affinity purification, ion exchange, and molecular sieves according to the properties of the protein. Help you to obtain the preliminary purified protein, and then obtain high-purity pure protein by HPLC, which can meet the requirements of various subsequent experiments.
Principles of Separation and Purification
SDS-PAGE is often used in scientific research work such as protein expression analysis. It can be used to obtain the electrophoresis pattern of protein samples, analyze or purify the samples in combination with other protein identification services based on mass spectrometry, and use in the protein spectrum analysis of complex biological samples. Creative Proteomics can provide protein separation services based on 1D SDS-PAGE or 2D SDS-PAGE according to your needs.
With the progress of sample background information, analysis methods, and sample preparation and extraction steps, a three-step purification strategy can be used to purify samples. This strategy is used to help develop the purification process of therapeutic proteins in the pharmaceutical industry, and it is equally effective in helping research laboratories develop purification schemes.
Creative Proteomics can provide you with the separation and purification service of known or unknown proteins in venom. Reasonable selection and combination of purification technologies are important.
Protein properties | Technology |
---|---|
Charge | Ion exchange (IEX) |
Size | Gel filtration (GF) |
Hydrophobicity |
Hydrophobic interaction (HIC) Reverse Action (RPC) |
Biometrics (specific ligand) | Affinity (AC) |
Charge, specific ligand or hydrophobicity |
Expanded bed adsorption (EBA) According to ion exchange Affinity or hydrophobicity |
If you don’t know anything about the target protein, use ion exchange—hydrophobic chromatography—gel filtration chromatography. This combination of technologies is considered a standard purification step.
Please contact us for more information.