Venom Glands mRNA and DNA Extraction

Venom gland is a general term for glands that secrete substances that are toxic to other animals. The venom secreted by the venom gland can be injected into the body of other animals or people through the bristles (such as spiny moth larvae), stings (such as spiders), tail spines (such as scorpions), and fangs (such as poisonous snakes) to cause poisoning. Due to different animals, the location and structure of the venom glands are also different.

Treatment of Venom Glands

Before the venom gland extraction, the stored venom of the animal needs to be removed to induce the RNA expression of the venom gland. And then the animal was dissected, the venom gland was stripped, and immediately frozen in liquid nitrogen for the extraction of total RNA from the venom gland tissue.

DNA and RNA Extraction

Creative Proteomics provides an efficient magnetic bead method for nucleic acid extraction. The extraction steps mainly include: lysis, binding, washing, and separation. The venom gland tissue releases DNA/RNA under the action of the lysis solution, and the surface-modified magnetic beads specifically bind to the released DNA/RNA to form a nucleic acid-magnetic bead complex. The impurities are washed away with the washing solution, and the nucleic acid is still adsorbed on the surface of the magnetic beads. Finally, the nucleic acid is separated from the magnetic beads to obtain a nucleic acid solution, which can be used for subsequent operations.

Purity and Integrity Testing

Obtaining high-quality, high-purity DNA or RNA samples is essential for subsequent experiments. Therefore, the identification of nucleic acids extracted from venom glands is also a necessary step.

Concentration/Purity Identification

Since the bases in nucleic acids all have conjugated double bonds, they have ultraviolet light absorption properties. Nucleic acid has a typical absorption curve in the ultraviolet spectral region, the absorption peak is at 260nm, and the absorption trough is at 230nm. The ultraviolet absorption spectrum data of nucleic acid is one of the important basic for identification of nucleic acid.

Integrity Testing

Agarose gel electrophoresis is routinely used to detect the size and integrity of nucleic acids. If there are degraded small DNA fragments, it can be prominently displayed on the electrophoresis graph. In the electrophoresis pattern of intact RNA or with little degradation, except for the characteristic three bands, the fluorescence intensity of 28S (or 23S) RNA is generally about twice that of 18S (or 16S) RNA, otherwise it indicates RNA degradation.

Our Advantages

• Use a small amount of sample
• High purity of purification
• Fast separation
• High-throughput extraction
• Non-toxic, harmless and non-polluting

Creative Proteomics can help you complete a series of services from venom gland isolation to nucleic acid extraction, and help you obtain high-quality venom and related products for follow-up research.

Please contact us for more information.