Subcellular Proteomics Service

Subcellular Proteomics

Nuclear-encoded proteins distribution and compartments development inside cells enables specific cellular functions. The exploration of the proteome of the cell in terms of the collection of its subcompartments is therefore both a practical approach and a necessary function assay. It demonstrates that proper interpretation of proteomic data requires information about compartmentation of protein machinery.

Subcellular proteomics decreases the complexity of proteomie discovery. With the typical compartment representing 500-4000 proteins, gel and MS based technique assist to reach a high-level of analytical resolution. In contrast, whole cell proteomes of 12, 000-40, 000 proteins extend well beyond the ability of proteomic tools to resolve them, leaving the studies being “tip of the iceberg” activities. Subcellular proteomics stands on the shoulders of decades of biochemical research that has developed methods for isolation of subcellular compartments. Extensive laboratory work involving the tinkering with density, size, and charge separation techniques has enabled incremental limitation of contamination in isolation methods from a range of subcellular structures.

Based on the powerful combination of subcellular fractionation and protein identification by LC-MS/MS, it enables us to develop the subcellular proteomics approach for the characterization of subcellular compartments.

Recent success stories demonstrated that the combination of subcellular prefractionation methods with proteomic analysis seems to be an potent approach to simplify complex protein extracts from cells or tissues and then facilitate low abundance proteins detection. It revealed that sophisticated strategies encompassing sample preparation, analytics and validation steps were required to fully exploit the potential of subcellular proteomics.

Our Subcellular Proteomics Service

Creative Proteomics uses Thermo Fisher's Orbitrap Fusion Lumos mass spectrometry platform combined with nanoLC-MS/MS nanoliter chromatography to provide subcellular proteomics analysis services. Combining years of experience in proteomics research, we have successfully achieved qualitative and quantitative analysis of multiple subcellular organelle components, including membrane proteome, chloroplast proteome, mitochondrial proteome, etc.

Biological membranes are the basic structures of cells and assume important functions in almost all life activities, which are mainly realized through membrane proteins. Abnormal functions of membrane proteins are directly related to many diseases, 50% of which are the targets of currently known drugs. Membrane proteomics can be used to find disease markers, screen drug targets, etc.

Chloroplasts are the main site of photosynthesis in plants and are one of the organelles containing genetic material in addition to the nucleus. Chloroplast proteomics is important for elucidating the laws of chloroplasts in plant growth and development and metabolic regulation and other life activities.

Mitochondria are the regulatory centers of cellular energy metabolism, biosynthesis and cell death, and changes in their protein structure and function can lead to the development of many diseases. Mitochondrial proteomics studies can provide an important theoretical basis for analyzing the physiological functions of mitochondria, exploring the pathogenesis of mitochondria-related diseases and promoting mitochondria-targeted drug development.

Exosomes are microsecretory vesicles, which are one of the products of intracellular vesicular transport and play an important role in physiopathological processes such as antigen presentation, tumor growth and migration, and repair of tissue damage. The exosome proteomics may facilitate further studies of exosome-related mechanisms and clinical applications.

Subcellular Proteomics Service Workflow

Technical Advantages

Highly stable protein extraction method: Optimized extraction protocol to increase extraction purity by 10-20% and protein throughput by 15%

High-performance mass spectrometry system: Equipped with a high-performance mass spectrometry system and full-scan acquisition method of of SWATH, which effectively enhances the identification of subcellular proteins with very low abundance

Multi-database integration: By integrating multiple relevant high-quality protein databases, the accuracy and throughput of subcellular protein localization is greatly improved

Multi-dimensional data analysis: In addition to comparing the identified proteins with more relevant subcellular databases, multiple software programs are used for predictive analysis of subcellular localization based on computational methods to further increase the identification throughput.

Creative Proteomics also provide the following bioinformatics services in Subcellular Proteomics:

Please feel free to Contact Us to discuss your projects. We hope you will find that we can meet your research needs.

Proteomics Sample Submission Guidelines

Ensure your samples are prepared and submitted correctly by downloading our comprehensive Proteomics Sample Submission Guidelines. This document provides detailed instructions and essential information to facilitate a smooth submission process. Click the link below to access the PDF and ensure your submission meets all necessary criteria.

Download

* For Research Use Only. Not for use in diagnostic procedures.

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From Our Clients

"I recently used their proteomics service for a project analyzing protein interactions in yeast models. The team was very responsive and helped clarify the methodology they employed, which made me feel confident in the results. The data quality was solid, with clear identification of several key proteins involved in our study. Their thorough analysis enabled me to pinpoint specific interactions that I hadn't considered before, which significantly improved the direction of my research. I appreciate their professionalism and support throughout the process."

Sarah Thompson, University of California, Berkeley

"Our lab collaborated with them on a project studying cancer biomarkers. The proteomics analysis provided was detailed and focused, specifically highlighting the differential expression of proteins between healthy and tumor samples. Their clear explanations of the data helped my team understand the biological implications. I also appreciated their willingness to revise the reports based on our feedback, ensuring that we had everything we needed for our publication. This collaborative spirit was invaluable."

Emily Rodriguez, Stanford University

"Our lab worked with them on a project studying the effects of diet on gut microbiota using proteomics. They used a label-free quantification method to analyze proteins in fecal samples before and after dietary intervention. The results showed significant changes in protein expression linked to microbial activity. This was pivotal for our hypothesis about diet-microbiota interactions. The clarity of their data presentation made it easy for our team to integrate these findings into our ongoing research."

Dr. Lisa Wong, University of Toronto

"My experience with Creative Proteomics during the mass spectrometry analysis was excellent. We sent in human saliva and mouse brain tissue samples, which they expertly analyzed using both LC-MS and GC-MS techniques. The results were invaluable, revealing key metabolites in the saliva and identifying biomarkers linked to brain function in the brain tissue."

Dr. Emily Carter, Senior Research Scientist

"The overall service from Creative Proteomics was outstanding. They made the entire process seamless and efficient, allowing us to focus on our research. We worked with leaf and root samples from various Arabidopsis genotypes for targeted metabolomics analysis. Their thorough profiling of primary and secondary metabolites gave us important insights into how the plants respond metabolically to environmental stress."

Dr. Laura Henderson, Plant Physiologist

"We had a pleasant collaboration with Creative Proteomics on mass spectrometry analysis of lipids. They conducted a detailed analysis of lipid species, providing us with important insights into lipid metabolism and its relationship with metabolic syndrome disease states."

Dr. Sarah Mitchell, Research Scientist

Online Inquiry

Please submit a detailed description of your project. We will provide you with a customized project plan to meet your research requests. You can also send emails directly to for inquiries.

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