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Protein Sample Preparation

Proteome is a collection of gene products expressed at both low levels and high levels. Protein sample preparation is a critical step in proteomic study because the quality and reproducibility of protein extraction significantly impacts the downstream identification efficiency and capability of mass spectrometers. Reliable and quantitative comparison of low-abundant proteins is a even more tremendous challenge of proteomic analysis.

Proteome is a very complex system. Unlike DNA and RNA, both of which are chemically homogeneous, proteins are very heterogeneous and vary widely with respect to their size, charge, hydrophobicity, and structure. Highly abundant proteins such as actin and tubulin or human serum albumin, proteins of the complement system and antibodies will comprise a significant portion of this homogenate, and the less-abundant regulatory proteins that are most informative regarding cellular response can be detected more efficiently with proper pretreatment and handling. In addition, many proteins undergo post-translational modification and may have a variety of non-peptide moieties bound to them. This chemical heterogeneity makes isolating proteins extremely challenging. So far, there has no standard method for preparing protein samples in proteomics study. Protocols differ regarding the source of samples, physical properties and subcellular location of the target proteins, protein abundance, and matrix effects, etc.

Scientists at Creative Proteomics recognize the need for customized sample preparation procedure, and continually strive to develop sample preparation methods that can help to solve various requirements of our customers.

Introduction of protein sample preparation

Our protein sample preparation service include:

Tissue & cell homogenization
Sample cleanup (remove DNA & RNA, s-s bond reduction, protein)
Depletion of high-abundance proteins from samples, like plasma
Protein extraction
Protein purification
Protein concentration
Protein digestion

Advantage of our protein sample preparation service:

Cutting-edge platform with ultrafiltration technology
Experienced scientists dealing with various samples
Optimized and quality control workflow to work with various samples
Project design based on the properties of target proteins

Ordering Procedure:

Ordering Procedure

Proteomics Sample Submission Guidelines

Ensure your samples are prepared and submitted correctly by downloading our comprehensive Proteomics Sample Submission Guidelines. This document provides detailed instructions and essential information to facilitate a smooth submission process. Click the link below to access the PDF and ensure your submission meets all necessary criteria.

Download

* For Research Use Only. Not for use in diagnostic procedures.

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From Our Clients

"I recently used their proteomics service for a project analyzing protein interactions in yeast models. The team was very responsive and helped clarify the methodology they employed, which made me feel confident in the results. The data quality was solid, with clear identification of several key proteins involved in our study. Their thorough analysis enabled me to pinpoint specific interactions that I hadn't considered before, which significantly improved the direction of my research. I appreciate their professionalism and support throughout the process."

Sarah Thompson, University of California, Berkeley

"Our lab collaborated with them on a project studying cancer biomarkers. The proteomics analysis provided was detailed and focused, specifically highlighting the differential expression of proteins between healthy and tumor samples. Their clear explanations of the data helped my team understand the biological implications. I also appreciated their willingness to revise the reports based on our feedback, ensuring that we had everything we needed for our publication. This collaborative spirit was invaluable."

Emily Rodriguez, Stanford University

"Our lab worked with them on a project studying the effects of diet on gut microbiota using proteomics. They used a label-free quantification method to analyze proteins in fecal samples before and after dietary intervention. The results showed significant changes in protein expression linked to microbial activity. This was pivotal for our hypothesis about diet-microbiota interactions. The clarity of their data presentation made it easy for our team to integrate these findings into our ongoing research."

Dr. Lisa Wong, University of Toronto

"My experience with Creative Proteomics during the mass spectrometry analysis was excellent. We sent in human saliva and mouse brain tissue samples, which they expertly analyzed using both LC-MS and GC-MS techniques. The results were invaluable, revealing key metabolites in the saliva and identifying biomarkers linked to brain function in the brain tissue."

Dr. Emily Carter, Senior Research Scientist

"The overall service from Creative Proteomics was outstanding. They made the entire process seamless and efficient, allowing us to focus on our research. We worked with leaf and root samples from various Arabidopsis genotypes for targeted metabolomics analysis. Their thorough profiling of primary and secondary metabolites gave us important insights into how the plants respond metabolically to environmental stress."

Dr. Laura Henderson, Plant Physiologist

"We had a pleasant collaboration with Creative Proteomics on mass spectrometry analysis of lipids. They conducted a detailed analysis of lipid species, providing us with important insights into lipid metabolism and its relationship with metabolic syndrome disease states."

Dr. Sarah Mitchell, Research Scientist

Online Inquiry

Please submit a detailed description of your project. We will provide you with a customized project plan to meet your research requests. You can also send emails directly to for inquiries.

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