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LC-MS/MS Untargeted Metabolomics

Metabolomics is an important area of study in systems biology, following genomics, transcriptomics, and proteomics. It is currently a research hotspot in the field of histology. The concept of metabolomics was introduced by Nicholson's group in the UK when they analyzed the composition of rat urine from a toxicology perspective. Metabolomics is a technique used to study the metabolic pathways of biological systems by examining changes in metabolites after a perturbation or stimulation, such as a specific gene variation or environmental change.

One such untargeted metabolomics technique is LC-MS/MS untargeting. This technique detects dynamic changes in a large number of metabolites before and after perturbation or stimulation of an organism by liquid chromatography-mass spectrometry (LC-MS) methods. It allows identification of metabolites with differential expression and elucidation of the organism's metabolism-related processes.

Creative Proteomics' LC-MS/MS untargeted metabolomics provides high throughput, high resolution, and high sensitivity for the analysis of metabolites that are difficult to volatilize or have poor thermal stability. This technique uses ultra-high pressure liquid chromatography (UPLC) or ultra-high performance liquid chromatography, which offers higher separation capacity, faster separation speed, and better sensitivity by using smaller particle size packing materials in combination with higher pressure.

In tandem mass spectrometry MS scan mode, primary and secondary mass spectral information of metabolites can be simultaneously acquired. This combined with metabolomics data processing software allows for resolution of mass spectral information, enabling the detection and identification of hundreds to thousands of metabolites in a single analysis.

Technical Advantages

Strict QC

Adopt the most stringent QC system to ensure the qualitative and quantitative results of each project

advanced platform

Based on high-resolution, high-sensitivity mass spectrometry platform

Application

Basic medicine, clinical diagnosis: biomarker Biomarker, disease mechanism, disease typing, personalized treatment, etc.

Biomedicine: drug mechanism of action, drug efficacy evaluation, drug development, etc.

Microbiology: pathogenesis, drug resistance mechanism, pathogen-host interaction research, etc.

Marine aquaculture: fishery resources, mariculture, fishery environment and aquatic product safety, etc.

Bioenergy and environmental science: fermentation process optimization, biofuel production, environmental risk assessment, etc.

Food nutrition: food storage and processing conditions optimization, food components and quality identification, functional food development, food safety monitoring and testing, etc.

Figure 1. The work flow of untargeted LC-MS-based metabolomics. Source (Chi Chen et al., Computational and Structural Biotechnology 2013)

Sample Requirements

Serum/plasma/urine/rumen fluid/cerebrospinal fluid/amniotic fluid and other homogeneous liquid samples: 200ul or more/sample; cerebrospinal fluid can be as low as 100ul

Animal tissue/faecal related samples: 200mg or more/sample.

Plant roots/stems/leaves/fruits/seeds: 200mg wet weight or more/sample

Cells/microorganisms/cell supernatant/culture supernatant: more than 10^7 cells or bacteria/sample; more than 2mL/sample of supernatant.

For other sample types, please inquire us.

Platform

Thermo QE/QE Plus, AB Sciex TripleTOF® 5600/6600, Waters VION Qtof , etc.

Metabolomics Sample Submission Guidelines

Download our Metabolomics Sample Preparation Guide for essential instructions on proper sample collection, storage, and transport for optimal experimental results. The guide covers various sample types, including tissues, serum, urine, and cells, along with quantity requirements for untargeted and targeted metabolomics.

Download

Metabolomics Sample Submission Guidelines

* For Research Use Only. Not for use in diagnostic procedures.

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From Our Clients

"I recently used their proteomics service for a project analyzing protein interactions in yeast models. The team was very responsive and helped clarify the methodology they employed, which made me feel confident in the results. The data quality was solid, with clear identification of several key proteins involved in our study. Their thorough analysis enabled me to pinpoint specific interactions that I hadn't considered before, which significantly improved the direction of my research. I appreciate their professionalism and support throughout the process."

Sarah Thompson, University of California, Berkeley

"Our lab collaborated with them on a project studying cancer biomarkers. The proteomics analysis provided was detailed and focused, specifically highlighting the differential expression of proteins between healthy and tumor samples. Their clear explanations of the data helped my team understand the biological implications. I also appreciated their willingness to revise the reports based on our feedback, ensuring that we had everything we needed for our publication. This collaborative spirit was invaluable."

Emily Rodriguez, Stanford University

"Our lab worked with them on a project studying the effects of diet on gut microbiota using proteomics. They used a label-free quantification method to analyze proteins in fecal samples before and after dietary intervention. The results showed significant changes in protein expression linked to microbial activity. This was pivotal for our hypothesis about diet-microbiota interactions. The clarity of their data presentation made it easy for our team to integrate these findings into our ongoing research."

Dr. Lisa Wong, University of Toronto

"My experience with Creative Proteomics during the mass spectrometry analysis was excellent. We sent in human saliva and mouse brain tissue samples, which they expertly analyzed using both LC-MS and GC-MS techniques. The results were invaluable, revealing key metabolites in the saliva and identifying biomarkers linked to brain function in the brain tissue."

Dr. Emily Carter, Senior Research Scientist

"The overall service from Creative Proteomics was outstanding. They made the entire process seamless and efficient, allowing us to focus on our research. We worked with leaf and root samples from various Arabidopsis genotypes for targeted metabolomics analysis. Their thorough profiling of primary and secondary metabolites gave us important insights into how the plants respond metabolically to environmental stress."

Dr. Laura Henderson, Plant Physiologist

"We had a pleasant collaboration with Creative Proteomics on mass spectrometry analysis of lipids. They conducted a detailed analysis of lipid species, providing us with important insights into lipid metabolism and its relationship with metabolic syndrome disease states."

Dr. Sarah Mitchell, Research Scientist

Online Inquiry

Please submit a detailed description of your project. We will provide you with a customized project plan to meet your research requests. You can also send emails directly to for inquiries.

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