Submit Your Request Now

Protein Gel and Imaging Analysis

Gel electrophoresis is an important methodology employed for protein analysis, which contains many different techniques, including sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), isoelectric focusing (IEF), and so on. SDS-PAGE, the most common gel electrophoresis used for proteins, provides an easy method to estimate the molecular weight of proteins, assess the complexity of the sample or the purity of preparation, and monitor the fractions obtained during chromatographic or other purification procedures. Moreover, electrophoretic patterns are required by ICH Q6B for the analysis of biopharmaceuticals.

Protein Gel and Imaging Analysis at Creative Proteomics

Electrophoretic methods have been applied to protein research for many years and separate proteins according to different physicochemical parameters. At Creative Proteomics, we can provide various protein gel and imaging analysis.

1D SDS-PAGE and IEF Analysis

1D SDS-PAGE is a method that separates proteins by molecular weight, where the samples are weighed and dissolved in SDS. SDS-PAGE is widely used in protein analysis, such as protein size determination, protein identification, sample purity analysis, and so on. IEF is an electrophoretic technique to separate proteins based on isoelectric point (pI), which can resolve different proteins of similar size that cannot be resolved on standard SDS-PAGE gels.

2D Electrophoresis Analysis

2-DE is a powerful tool for separation and fractionation of complex protein mixtures. The 2-DE separates protein firstly by IEF and then by SDS-PAGE. At Creative Proteomics, we can provide 2D electrophoresis services according to standard operating procedures (SOPs). High resolution 2D electrophoresis is used for protein species and isoform distribution analysis, host cell protein analysis, quantitative comparison of cell states, and so on.

2-DE /SDS-PAGE Protein Identification by LC-MS/MS

Creative Proteomics uses LC-MS/MS technology to be able to efficiently and accurately identify proteins in SDS-PAGE and 2-DE samples, and optimize the in-gel digestion method to significantly improve the success rate of protein mass spectrometry identification and peptide coverage.

Western Blot & Electrical Transfer Analysis

Western blot is a commonly used method for protein analysis, such as qualitative and semi-quantitative analysis. In this method, proteins are separated by 2-DE and then these proteins are transferred to a carrier membrane. Western blot can be used for the qualitative detection of single proteins and protein modifications.

2D Blue Native / SDS-PAGE for Complex Analysis

Blue native (BN) PAGE, one of the native gel methods, can analyze proteins that are in a folded state. In 2D BN/SDS-PAGE analysis, proteins are analyzed by dimension BN-PAGE and second dimension SDS-PAGE. 2D BN/SDS-PAGE has been proved to be a useful tool for protein complex analysis. It can provide information about the size, number, protein composition, stoichiometry and relative abundance of the sample. Creative Proteomics provides a method for the global analysis of the subunits of protein complexes through 2D Blue Native / SDS-PAGE.

Isoelectric Point Analysis

The isoelectric point is defined as the pH at which the net charge of the protein becomes zero. Isoelectric point analysis can be used to study heterogeneity, analyze protein folding status, and predict protein-protein interactions.

Imaging Analysis

Imaging analysis is an important part of protein gel that can provide various information, including the novel or missing detection, protein spots quantification, pI and Mr values determination, etc. By using a scanner or camera with computer software, the gel images can be converted into digital data and the complex 2-D patterns can be evaluated.

Our Advantages

A range of techniques for various applications, including molecular weight measurement, impurities characterization, protein expression research, and so on.
Different samples, including body fluids, tissues, blood cells, cell cultures, microorganisms, plants, and so on.
Different gels sizes including 8x7 cm, 23x30cm, 40x30cm and 60x30cm.

Based on professional knowledge and experienced staff, Creative Proteomics provides a wide range of protein gel and imaging analysis services in a time-saving and cost-efficient manner. Our ordering procedure is as follows. If you have any questions or specific requirements, please feel free to contact us.

Ordering Procedure

Reference

Garfin D E. Gel electrophoresis of proteins. Essential cell biology, 2003, 1(7): 197-268.

Proteomics Sample Submission Guidelines

Ensure your samples are prepared and submitted correctly by downloading our comprehensive Proteomics Sample Submission Guidelines. This document provides detailed instructions and essential information to facilitate a smooth submission process. Click the link below to access the PDF and ensure your submission meets all necessary criteria.

Download

* For Research Use Only. Not for use in diagnostic procedures.

Our customer service representatives are available 24 hours a day, 7 days a week. Inquiry

From Our Clients

"I recently used their proteomics service for a project analyzing protein interactions in yeast models. The team was very responsive and helped clarify the methodology they employed, which made me feel confident in the results. The data quality was solid, with clear identification of several key proteins involved in our study. Their thorough analysis enabled me to pinpoint specific interactions that I hadn't considered before, which significantly improved the direction of my research. I appreciate their professionalism and support throughout the process."

Sarah Thompson, University of California, Berkeley

"Our lab collaborated with them on a project studying cancer biomarkers. The proteomics analysis provided was detailed and focused, specifically highlighting the differential expression of proteins between healthy and tumor samples. Their clear explanations of the data helped my team understand the biological implications. I also appreciated their willingness to revise the reports based on our feedback, ensuring that we had everything we needed for our publication. This collaborative spirit was invaluable."

Emily Rodriguez, Stanford University

"Our lab worked with them on a project studying the effects of diet on gut microbiota using proteomics. They used a label-free quantification method to analyze proteins in fecal samples before and after dietary intervention. The results showed significant changes in protein expression linked to microbial activity. This was pivotal for our hypothesis about diet-microbiota interactions. The clarity of their data presentation made it easy for our team to integrate these findings into our ongoing research."

Dr. Lisa Wong, University of Toronto

"My experience with Creative Proteomics during the mass spectrometry analysis was excellent. We sent in human saliva and mouse brain tissue samples, which they expertly analyzed using both LC-MS and GC-MS techniques. The results were invaluable, revealing key metabolites in the saliva and identifying biomarkers linked to brain function in the brain tissue."

Dr. Emily Carter, Senior Research Scientist

"The overall service from Creative Proteomics was outstanding. They made the entire process seamless and efficient, allowing us to focus on our research. We worked with leaf and root samples from various Arabidopsis genotypes for targeted metabolomics analysis. Their thorough profiling of primary and secondary metabolites gave us important insights into how the plants respond metabolically to environmental stress."

Dr. Laura Henderson, Plant Physiologist

"We had a pleasant collaboration with Creative Proteomics on mass spectrometry analysis of lipids. They conducted a detailed analysis of lipid species, providing us with important insights into lipid metabolism and its relationship with metabolic syndrome disease states."

Dr. Sarah Mitchell, Research Scientist

Online Inquiry

Please submit a detailed description of your project. We will provide you with a customized project plan to meet your research requests. You can also send emails directly to for inquiries.

Great Minds Choose Creative Proteomics