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Bioanalysis of DNA Methylations

DNA methylation is a process by which the methyl groups are covalently transferred to cytosine & adenine of DNA nucleotides (adenine methylation is restricted to prokaryotes), by the catalysis of DNA methyltransferases. Typically acting to suppress gene transcription, DNA methylation is essential for normal development and associated with a series of key processes and biological activities, including genomic imprinting, X-chromosome inactivation, suppression of repetitive elements, and carcinogenesis. In mammals, insects, bacteria, fungi and plants, DNA methylation is widely detected in the species above; DNA methylation typically occurs at the 5 position of cytosine in a CpG dinucleotide contect, which can selectively reduce specific gene expression; non-CpG methylation is prevalent in embryonic stem cells, and has also been indicated in neural development.

As one of the major epigenetic processes, it is widely accepted that there is an underlying relationship between DNA methylation, chromatin structure & human malignancies. Because of the significance of DNA methylation as biomarkers in cancer molecular diagnostics, aging, atherosclerosis and other diseases, currently several analytic have been developed likely chemical assay or biochips. Now with the development of mass spectrometry, LC-MS/MS can work as a sensitive and reliable analytic method to globally profile methylation with high throughput, ultra-sensitivity and high confidence. Oligonucleotides containing unusual modifications can therefore be verified by mass spectrometery rapidly. And the typical work-flow for LC-MS based analysis would be as follow:

HPLC can be used to separate the different deoxyribonucleotide fragments in a base-specific digested sample of DNA, yielding different retention times on the chromatographic column;
In tandem mass spectrometry, the protonated deoxyribonucleotides are sequentially selected by the first mass analyzer, and fragmented in the collision cell to generate product ions;
The patterns of fragment ions from the selected precursor would be scanned by the second mass analyzer. The bioinformatic tools would be applied for identifications of DNA sequence fragments and DNA methylation.

Creative Proteomics have developed valid SOPs of DNA isolation and digestion, HPLC separation, and analysis with tandem mass spectrometry, based on the reliable protocols from reputable journals. Operating the advanced and integrated facilities, our bioanalytical staff can generate robust analytic results for your project.

Proteomics Sample Submission Guidelines

Ensure your samples are prepared and submitted correctly by downloading our comprehensive Proteomics Sample Submission Guidelines. This document provides detailed instructions and essential information to facilitate a smooth submission process. Click the link below to access the PDF and ensure your submission meets all necessary criteria.

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* For Research Use Only. Not for use in diagnostic procedures.

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From Our Clients

"I recently used their proteomics service for a project analyzing protein interactions in yeast models. The team was very responsive and helped clarify the methodology they employed, which made me feel confident in the results. The data quality was solid, with clear identification of several key proteins involved in our study. Their thorough analysis enabled me to pinpoint specific interactions that I hadn't considered before, which significantly improved the direction of my research. I appreciate their professionalism and support throughout the process."

Sarah Thompson, University of California, Berkeley

"Our lab collaborated with them on a project studying cancer biomarkers. The proteomics analysis provided was detailed and focused, specifically highlighting the differential expression of proteins between healthy and tumor samples. Their clear explanations of the data helped my team understand the biological implications. I also appreciated their willingness to revise the reports based on our feedback, ensuring that we had everything we needed for our publication. This collaborative spirit was invaluable."

Emily Rodriguez, Stanford University

"Our lab worked with them on a project studying the effects of diet on gut microbiota using proteomics. They used a label-free quantification method to analyze proteins in fecal samples before and after dietary intervention. The results showed significant changes in protein expression linked to microbial activity. This was pivotal for our hypothesis about diet-microbiota interactions. The clarity of their data presentation made it easy for our team to integrate these findings into our ongoing research."

Dr. Lisa Wong, University of Toronto

"My experience with Creative Proteomics during the mass spectrometry analysis was excellent. We sent in human saliva and mouse brain tissue samples, which they expertly analyzed using both LC-MS and GC-MS techniques. The results were invaluable, revealing key metabolites in the saliva and identifying biomarkers linked to brain function in the brain tissue."

Dr. Emily Carter, Senior Research Scientist

"The overall service from Creative Proteomics was outstanding. They made the entire process seamless and efficient, allowing us to focus on our research. We worked with leaf and root samples from various Arabidopsis genotypes for targeted metabolomics analysis. Their thorough profiling of primary and secondary metabolites gave us important insights into how the plants respond metabolically to environmental stress."

Dr. Laura Henderson, Plant Physiologist

"We had a pleasant collaboration with Creative Proteomics on mass spectrometry analysis of lipids. They conducted a detailed analysis of lipid species, providing us with important insights into lipid metabolism and its relationship with metabolic syndrome disease states."

Dr. Sarah Mitchell, Research Scientist

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