Submit Your Request Now

Plant Untargeted Lipidomics

Lipids play important roles in plants, serving as structural components of cellular membranes, major storage reserve in seeds, pigments for energy capture in leaves, and signaling molecules for cellular communication.

With the development of lipidomics, the applications of mass spectrometric analysis of plant lipids also improved significantly. "Plant lipidomics" is defined as the analysis of plant cellular lipids using mass spectrometry method. A variety of methods, including ESI-MS, ESI-MS/MS, GC-MS are used in plant lipidomics studies. Untargeted lipid profiling can be used to detect metabolic changes in response to developmental and environmental changes.

Plant Untargeted Lipidomics Figure 1. Workflow of plant lipidomics analysis

Lipids are shown to play important roles in plant growth, development, and responses to environmental changes. Glycerolipids are the most abundant lipid type in plants, comprising phospholipids, glycolipids and neutral lipids. Phospholipids can be further divided into subgroups including phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), phosphatidylglycerol (PG) and phosphatidylinositol (PI). Glycolipid group includes monogalactosyldiaclyglycerol (MGDG), digalactosyl diacylglycerol (DGDG) and sulphoquinovosyl diacylglycerol (SQDG). The composition of these compounds differs among different tissues and species of the plants, and is also strongly influenced by nutrients and environments.

Untargeted lipidomics can be used to study many research topics including:

Stress-induced change of plant lipidomics.
Injury-induced change of plant lipidomics.
Development and aging induced change of plant lipidomics.

The instrumentation useful for the identification of plant lipidomics includes MS, GC, HPLC, or LC-MS, GC-MS. The coupling of chromatographic methods such as GC or HPLC with MS can greatly increase coverage of lipids. As one of the widely used analytical techniques in plant lipidomics, GC-MS is utilized to qualitatively and quantitatively analyze a wide range of volatile and derivatized nonvolatile lipids ranging from 14-24 carbons which contains 0-3 double bonds. Compared to LC-MS and LC-NMR, GC-MS is of analytical reproducibility and lower costs. LC-MS has also been used in these years for the untargeted large-scale analysis of plant lipids.

With integrated set of separation, characterization, identification and quantification systems featured with excellent robustness & reproducibility, high and ultra-sensitivity, Creative Proteomics provides reliable, rapid and cost-effective plant untargeted lipidomics analysis with LC-MS, GC-MS.

Sample Requirement

Normal Volume: 200ul plasma; 100mg tissue; 2e7 cells
Minimal Volume: 50uL plasma; 50mg tissue; 5e6 cells

How to place an order:

ow To Place your Order

Lipidomics Sample Submission Guidelines

Download our Lipidomics Sample Preparation Guide for essential instructions on proper sample collection, storage, and transport for optimal experimental results. The guide covers various sample types, including tissues, serum, urine, and cells, along with quantity requirements for untargeted and targeted lipidomics.

Download

Metabolomics Sample Submission Guidelines

* For Research Use Only. Not for use in diagnostic procedures.

Our customer service representatives are available 24 hours a day, 7 days a week. Inquiry

From Our Clients

"I recently used their proteomics service for a project analyzing protein interactions in yeast models. The team was very responsive and helped clarify the methodology they employed, which made me feel confident in the results. The data quality was solid, with clear identification of several key proteins involved in our study. Their thorough analysis enabled me to pinpoint specific interactions that I hadn't considered before, which significantly improved the direction of my research. I appreciate their professionalism and support throughout the process."

Sarah Thompson, University of California, Berkeley

"Our lab collaborated with them on a project studying cancer biomarkers. The proteomics analysis provided was detailed and focused, specifically highlighting the differential expression of proteins between healthy and tumor samples. Their clear explanations of the data helped my team understand the biological implications. I also appreciated their willingness to revise the reports based on our feedback, ensuring that we had everything we needed for our publication. This collaborative spirit was invaluable."

Emily Rodriguez, Stanford University

"Our lab worked with them on a project studying the effects of diet on gut microbiota using proteomics. They used a label-free quantification method to analyze proteins in fecal samples before and after dietary intervention. The results showed significant changes in protein expression linked to microbial activity. This was pivotal for our hypothesis about diet-microbiota interactions. The clarity of their data presentation made it easy for our team to integrate these findings into our ongoing research."

Dr. Lisa Wong, University of Toronto

"My experience with Creative Proteomics during the mass spectrometry analysis was excellent. We sent in human saliva and mouse brain tissue samples, which they expertly analyzed using both LC-MS and GC-MS techniques. The results were invaluable, revealing key metabolites in the saliva and identifying biomarkers linked to brain function in the brain tissue."

Dr. Emily Carter, Senior Research Scientist

"The overall service from Creative Proteomics was outstanding. They made the entire process seamless and efficient, allowing us to focus on our research. We worked with leaf and root samples from various Arabidopsis genotypes for targeted metabolomics analysis. Their thorough profiling of primary and secondary metabolites gave us important insights into how the plants respond metabolically to environmental stress."

Dr. Laura Henderson, Plant Physiologist

"We had a pleasant collaboration with Creative Proteomics on mass spectrometry analysis of lipids. They conducted a detailed analysis of lipid species, providing us with important insights into lipid metabolism and its relationship with metabolic syndrome disease states."

Dr. Sarah Mitchell, Research Scientist

Online Inquiry

Please submit a detailed description of your project. We will provide you with a customized project plan to meet your research requests. You can also send emails directly to for inquiries.

Great Minds Choose Creative Proteomics