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Sphingomyelin Profiling Service

Sphingomyelin profiling service provides data on all individual molecular species. Sphingomyelins (SM) constitute an important class of phospholipids in the membranes of most eukaryotic cells. In mammalian tissues, SM usually constitute 2–15% of the total organ phospholipid, but certain tissues such as brain, peripheral nervous tissue and ocular lenses have even higher SM contents, especially in some nerve cell axons. Sphingomyelin (or ceramide phosphorylcholine) consists of a ceramide unit with a phosphorylcholine moiety attached. Sphingomyelin does not appear to occur in plants or fungi, which produce ceramide phosphorylinositol instead, or in bacteria with rare exceptions, and its evolutionary significance is a matter for speculation.

Sphingomyelin profiling service provides data on all individual molecular species. We recommend these profiling scans at the semi-quantitative level, which allows direct comparison between samples or treatments. Sphingomyelin usually isolated from animal tissues by TLC or HPLC. The peaks or bands usually split into two or three resolved fractions, due to the changes in hydrophobicity resulting from the wide range of chain lengths in the fatty acid constituents, or to the presence of 2-hydroxy acids. Molecular species of the intact lipid can be resolved by reversed-phase HPLC, but another useful approach is to hydrolyse to the less polar ceramides with the enzyme phospholipase C, analysed the ceramides instead. Sphingomyelin profiling service at Creative Proteomics ESI Liquid Chromatography Mass Spectrometry (ESI- LC/MS) is used for the analysis of sphingomyelin and other sphingo-phospholipids.

Technology Platform:

ESI Liquid Chromatography Mass Spectrometry (ESI- LC/MS)

Sample Type:

Cultured Cells
Cultured or uncultured primary mouse cells or cell lines

Sphingomyelins could monitored in This Service
14:0 SM	15:0 SM	16:1 SM
16:0 SM	18:1 SM	18:0 SM
20:1 SM	20:0 SM	22:1 SM
22:0 SM	23:0 SM	24:2 SM
24:1 SM	24:0 SM	More on Request

Ordering Procedure:

Ordering Procedure

With integrated set of separation, characterization, identification and quantification systems featured with excellent robustness & reproducibility, high and ultra-sensitivity, Creative Proteomics provides reliable, rapid and cost-effective Lipidomics services.

Lipidomics Sample Submission Guidelines

Download our Lipidomics Sample Preparation Guide for essential instructions on proper sample collection, storage, and transport for optimal experimental results. The guide covers various sample types, including tissues, serum, urine, and cells, along with quantity requirements for untargeted and targeted lipidomics.

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Metabolomics Sample Submission Guidelines

* For Research Use Only. Not for use in diagnostic procedures.

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From Our Clients

"I recently used their proteomics service for a project analyzing protein interactions in yeast models. The team was very responsive and helped clarify the methodology they employed, which made me feel confident in the results. The data quality was solid, with clear identification of several key proteins involved in our study. Their thorough analysis enabled me to pinpoint specific interactions that I hadn't considered before, which significantly improved the direction of my research. I appreciate their professionalism and support throughout the process."

Sarah Thompson, University of California, Berkeley

"Our lab collaborated with them on a project studying cancer biomarkers. The proteomics analysis provided was detailed and focused, specifically highlighting the differential expression of proteins between healthy and tumor samples. Their clear explanations of the data helped my team understand the biological implications. I also appreciated their willingness to revise the reports based on our feedback, ensuring that we had everything we needed for our publication. This collaborative spirit was invaluable."

Emily Rodriguez, Stanford University

"Our lab worked with them on a project studying the effects of diet on gut microbiota using proteomics. They used a label-free quantification method to analyze proteins in fecal samples before and after dietary intervention. The results showed significant changes in protein expression linked to microbial activity. This was pivotal for our hypothesis about diet-microbiota interactions. The clarity of their data presentation made it easy for our team to integrate these findings into our ongoing research."

Dr. Lisa Wong, University of Toronto

"My experience with Creative Proteomics during the mass spectrometry analysis was excellent. We sent in human saliva and mouse brain tissue samples, which they expertly analyzed using both LC-MS and GC-MS techniques. The results were invaluable, revealing key metabolites in the saliva and identifying biomarkers linked to brain function in the brain tissue."

Dr. Emily Carter, Senior Research Scientist

"The overall service from Creative Proteomics was outstanding. They made the entire process seamless and efficient, allowing us to focus on our research. We worked with leaf and root samples from various Arabidopsis genotypes for targeted metabolomics analysis. Their thorough profiling of primary and secondary metabolites gave us important insights into how the plants respond metabolically to environmental stress."

Dr. Laura Henderson, Plant Physiologist

"We had a pleasant collaboration with Creative Proteomics on mass spectrometry analysis of lipids. They conducted a detailed analysis of lipid species, providing us with important insights into lipid metabolism and its relationship with metabolic syndrome disease states."

Dr. Sarah Mitchell, Research Scientist

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