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Metabolic Flux Analysis (MFA) Service

Metabolic flux analysis (MFA), an important analytical technique, is used to examine production and consumption rates of metabolites in a biological system. In systems metabolic engineering, MFA can be used for understanding cellular physiology under particular conditions and predicting its metabolic capability after genetic or environmental perturbations. MFA can also be used for the elucidation of cellular mechanisms. By using stable isotopes such as ²H, ¹³C, and ¹⁵N, MFA can identify and quantitatively estimate the metabolism through metabolic pathways by observing the position of the isotopic label along the metabolic pathways. ¹H and ¹³C NMR methods, as well as mass spectrometry methods, have been used for metabolic flux analysis. Recently, tandem mass spectrometry, emerged as a new analytical technique, aids to support additional information for high-resolution quantification of metabolic fluxes in complex biological systems.

Metabolic Flux Analysis (MFA)

Overview of Metabolomics Flux Analysis Service

Creative proteomics has developed a novel metabolic flux analysis platform, which allows for the determination of control structure and metabolic pathway regulation network. One of the promising advantages of our platform includes regulatory information crucial for understanding dynamic systems. This analytical platform is widely applicable to any metabolic system that can be depicted by kinetic modeling. Our approach is composed of several integrated quantitative methodologies, including a detailed computational dynamic model accounting for regulatory interactions.

Creative Proteomics provides unmatched metabolic flux analysis services to help accelerate your research. Our services include:

Experimental Design. We can design experiments tailed to meet your study objects and purposes.
Sample Analysis. We can provide analysis of general trends in metabolomic flux, such as glucose metabolic flux, glutamine flux, citric acid cycle (TCA cycle) metabolic flux, etc.
Data analysis. We provide raw data processing from MS, flux analysis using proprietary software to quantify fluxes and interpret results.

We have various methods for metabolic flux analysis, including but not limited to:

¹³C-metabolic flux analysis (¹³C-MFA)
¹³C dynamic metabolic flux analysis (¹³C-DMFA)
Dynamic metabolic flux analysis (DMFA)
Stoichiometric metabolic flux analysis
Isotopic non-stationary ¹³C metabolic (¹³C-NMFA)

Advantages of Metabolic Flux Analysis

At Creative Proteomics, whether you want to analyze the general trends in metabolomic flux or a given pathway, we can guarantee the data to meet your needs.

Employ the most sophisticated quantitative approaches to ensure the maximal coverage of pathways of interest
Obtain specific and reliable data for quantifying specific reactions with confidence
Discovery and implement high resolution approaches to flux analysis

Common Sample Types

Various types of cells, tissues and microbial organisms, culture media or fermentation broth.

Coverage Pathways

Glycolytic pathway
Tricarboxylic acid cycle pathway
Pentose phosphate pathway
Amino acid metabolism (Glutamine, Serine, Tryptophan, Leucine, lsoleucine and Valine, etc.)
Fatty acid metabolism
One-carbon metabolism (folic acid metabolism, methionine cycle)
Nucleotide metabolism
NADPH and other energy metabolic pathways

For other metabolic pathways or metabolites of interest, please contact us.

Based on high-performance quantitative techniques, Creative Proteomics can provide metabolic flux analysis solutions without compromising on data completeness. As every project has different requirements, please contact our specialists to discuss your specific needs. We are looking forward to cooperating with you.

Reference

Antoniewicz M R. Methods and advances in metabolic flux analysis: a mini-review. Journal of industrial microbiology & biotechnology, 2015, 42(3): 317-325.

Metabolomics Sample Submission Guidelines

Download our Metabolomics Sample Preparation Guide for essential instructions on proper sample collection, storage, and transport for optimal experimental results. The guide covers various sample types, including tissues, serum, urine, and cells, along with quantity requirements for untargeted and targeted metabolomics.

Download

Metabolomics Sample Submission Guidelines

* For Research Use Only. Not for use in diagnostic procedures.

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From Our Clients

"I recently used their proteomics service for a project analyzing protein interactions in yeast models. The team was very responsive and helped clarify the methodology they employed, which made me feel confident in the results. The data quality was solid, with clear identification of several key proteins involved in our study. Their thorough analysis enabled me to pinpoint specific interactions that I hadn't considered before, which significantly improved the direction of my research. I appreciate their professionalism and support throughout the process."

Sarah Thompson, University of California, Berkeley

"Our lab collaborated with them on a project studying cancer biomarkers. The proteomics analysis provided was detailed and focused, specifically highlighting the differential expression of proteins between healthy and tumor samples. Their clear explanations of the data helped my team understand the biological implications. I also appreciated their willingness to revise the reports based on our feedback, ensuring that we had everything we needed for our publication. This collaborative spirit was invaluable."

Emily Rodriguez, Stanford University

"Our lab worked with them on a project studying the effects of diet on gut microbiota using proteomics. They used a label-free quantification method to analyze proteins in fecal samples before and after dietary intervention. The results showed significant changes in protein expression linked to microbial activity. This was pivotal for our hypothesis about diet-microbiota interactions. The clarity of their data presentation made it easy for our team to integrate these findings into our ongoing research."

Dr. Lisa Wong, University of Toronto

"My experience with Creative Proteomics during the mass spectrometry analysis was excellent. We sent in human saliva and mouse brain tissue samples, which they expertly analyzed using both LC-MS and GC-MS techniques. The results were invaluable, revealing key metabolites in the saliva and identifying biomarkers linked to brain function in the brain tissue."

Dr. Emily Carter, Senior Research Scientist

"The overall service from Creative Proteomics was outstanding. They made the entire process seamless and efficient, allowing us to focus on our research. We worked with leaf and root samples from various Arabidopsis genotypes for targeted metabolomics analysis. Their thorough profiling of primary and secondary metabolites gave us important insights into how the plants respond metabolically to environmental stress."

Dr. Laura Henderson, Plant Physiologist

"We had a pleasant collaboration with Creative Proteomics on mass spectrometry analysis of lipids. They conducted a detailed analysis of lipid species, providing us with important insights into lipid metabolism and its relationship with metabolic syndrome disease states."

Dr. Sarah Mitchell, Research Scientist

Online Inquiry

Please submit a detailed description of your project. We will provide you with a customized project plan to meet your research requests. You can also send emails directly to for inquiries.

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