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Trimetlylamine Oxide (TMAO) Analysis Service

Trimethylamine oxide (Trimetlylamine oxide, TMAO) is a small molecule organic compound. In 2011, Cleveland Laboratory, one of the world's top cardiovascular disease medical centers, reported in Nature that TMAO, a metabolite of intestinal bacteria, is related to heart disease. , bringing the small molecule TMAO into the public view.

Targeted metabolomics can collect metabolome data of biological samples through MRM mode for target metabolites of interest to achieve qualitative analysis of metabolites. Combining the external standard quantitative method allows for the absolute quantification of metabolites in the sample using the standard curve of the standard.

TMAO is a low-molecular-weight amine compound with a relatively high content in aquatic products. It is also a metabolite related to intestinal flora. In the human body, intestinal bacteria can metabolize choline and lecithin to produce trimethylamine (TMA), which is then metabolized into TMAO. Trimethylamine oxide also exists in mammals, plants and fungi. Trimethylamine oxide and its metabolites participate in many important biological functions of the body, such as regulating osmotic pressure, stabilizing proteins, preventing protein denaturation, and participating in cell division. Studies have shown that elevated levels of TMAO are significantly positively correlated with cardiovascular diseases, and are closely related to hypertension, cardiac insufficiency, arteriosclerosis and other diseases. Therefore, trimethylamine oxide has received more and more attention. The study of trimethylamine oxide and related metabolites has important reference value in exploring TMAO metabolism, pathogenesis of related diseases, diagnosis, treatment and prevention methods.

Meat Biomarkers (TMAO)

Our TMAO Analysis Service

With integrated set of separation, characterization, identification and quantification systems featured with excellent robustness & reproducibility, high and ultra-sensitivity, Creative Proteomics provides reliable, rapid and cost-effective TMO targeted metabolomics services.

Creative Proteomics has established a robust, reproducible, and highly sensitive HPLC-MS platform for the simultaneous quantification of TMAO. Our high-throughput TMAO detection and analysis technology is based on LC-MS/MS and allows for the absolute quantitative detection of TMAO and its metabolites, including Trimethylamine N-oxide, Trimethylamine, Choline (Choline), Betaine, Creatinine, and L-carnitine. Complete quantitative analysis of TMAO and related metabolites can provide a reliable basis for the study of the etiology and pathology of related diseases.

Advantages

Advanced Platform: We utilize AB SCIEX QTRAP 6500+, a high-precision and high-resolution targeted detection mass spectrometer, to achieve precise and accurate targeted identification.

Self-built Database: We have independently built the TMAO metabolite database and provided all standard curves for measured substances.

Strict Quality Control: Our dual quality control system includes Standard QC, Sample QC, and exogenous internal standards to ensure data stability and accuracy.

Accurate Qualitative Results: Each compound has more than two pairs of characteristic ion pairs to ensure qualitative accuracy.

Accurate Quantitative Results: We use MRM mode with a triple quadrupole to accurately detect the relative content of substances.

Wide Linear Range: Our correlation coefficient is greater than 0.99, enabling us to perform quantitative analysis on different types of complex samples.

Application

Gut flora research

Cardiovascular research, renal disease research, etc.

Identification of neurotransmitters in samples such as tissue cells;

Research on the pathogenesis of nervous system diseases;

Workflow

Data Analysis

Analysis Type	Analysis Content
QC	QC RSD
Difference Analysis	Univariate Statistical Analysis T Test
Difference Analysis	Box Plots
Functional Analysis	Hierarchical Cluster Analysis
Functional Analysis	KEGG Analysis
Advanced Bioinformatics Analysis	Regression Analysis
	Trend Cluster Analysis
	Machine Learning ROC

Metabolites Quantified in This Service
Carnitine
Choline
Betaine
Creatinine
Trimethylamine (TMA)
Trimethylamine N-oxide (TMAO)

Sample Requirement

Blood (serum, plasma) ≥200 μL

Animal tissue ≥120 mg

Feces ≥200 mg

References

Long-Term Changes in Gut Microbial Metabolite Trimethylamine N-Oxide and Coronary Heart Disease Risk. Journal of the American College of Cardiology. 2020.
Development of a gut microbe–targeted nonlethal therapeutic to inhibit thrombosis potential. Nature Medicine, 2018.

Metabolomics Sample Submission Guidelines

Download our Metabolomics Sample Preparation Guide for essential instructions on proper sample collection, storage, and transport for optimal experimental results. The guide covers various sample types, including tissues, serum, urine, and cells, along with quantity requirements for untargeted and targeted metabolomics.

Download

Metabolomics Sample Submission Guidelines

* For Research Use Only. Not for use in diagnostic procedures.

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"I recently used their proteomics service for a project analyzing protein interactions in yeast models. The team was very responsive and helped clarify the methodology they employed, which made me feel confident in the results. The data quality was solid, with clear identification of several key proteins involved in our study. Their thorough analysis enabled me to pinpoint specific interactions that I hadn't considered before, which significantly improved the direction of my research. I appreciate their professionalism and support throughout the process."

Sarah Thompson, University of California, Berkeley

"Our lab collaborated with them on a project studying cancer biomarkers. The proteomics analysis provided was detailed and focused, specifically highlighting the differential expression of proteins between healthy and tumor samples. Their clear explanations of the data helped my team understand the biological implications. I also appreciated their willingness to revise the reports based on our feedback, ensuring that we had everything we needed for our publication. This collaborative spirit was invaluable."

Emily Rodriguez, Stanford University

"Our lab worked with them on a project studying the effects of diet on gut microbiota using proteomics. They used a label-free quantification method to analyze proteins in fecal samples before and after dietary intervention. The results showed significant changes in protein expression linked to microbial activity. This was pivotal for our hypothesis about diet-microbiota interactions. The clarity of their data presentation made it easy for our team to integrate these findings into our ongoing research."

Dr. Lisa Wong, University of Toronto

"My experience with Creative Proteomics during the mass spectrometry analysis was excellent. We sent in human saliva and mouse brain tissue samples, which they expertly analyzed using both LC-MS and GC-MS techniques. The results were invaluable, revealing key metabolites in the saliva and identifying biomarkers linked to brain function in the brain tissue."

Dr. Emily Carter, Senior Research Scientist

"The overall service from Creative Proteomics was outstanding. They made the entire process seamless and efficient, allowing us to focus on our research. We worked with leaf and root samples from various Arabidopsis genotypes for targeted metabolomics analysis. Their thorough profiling of primary and secondary metabolites gave us important insights into how the plants respond metabolically to environmental stress."

Dr. Laura Henderson, Plant Physiologist

"We had a pleasant collaboration with Creative Proteomics on mass spectrometry analysis of lipids. They conducted a detailed analysis of lipid species, providing us with important insights into lipid metabolism and its relationship with metabolic syndrome disease states."

Dr. Sarah Mitchell, Research Scientist

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