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Endocannabinoids Analysis Service

Endocannabinoids are amphiphilic molecules which are synthesized from phospholipids in the nervous system. Together with their G-protein coupled receptors they form the endocannabinoid system. The best studied endocannabinoids are the N-arachidonylethanolamide (anandamide) and the 2-arachidonoylglycerol (2-AG). They consist of an amide or an ester head, and linked with an arachidonyl chain. The physiological activities of Endocannabinoids mainly occur by binding to cannabinoid receptors or TRP channels. Moreover, it has been demonstrated that the endocannabinoids are involved in many membrane proteins in the presence of antagonists of their classical receptors, indicating the endocannabinoids can mediate physiological activities in a receptor-independent way.

Endocannabinoids Analysis Service Figure 1. Structure of endocannabinoids anandamide and 2-AG

The endocannabinoids have been shown to regulate a wide range of physiological and cognitive processes including fertility, pregnancy, during pre- and postnatal development, motor control, appetite, food intake, pain-sensation, mood, memory acquisition and learning. In humans, exercise has been shown to induce the increase of the endocannabinoids levels in plasma, mainly because endocannabinoids can effectively cross the blood–brain barrier, and contribute to the development of exercise-induced euphoria in humans. Disorders of the endocannabinoid system have been related to several neuro-inflammatory diseases such as Alzheimer, Parkinson, Huntington and Multiple Sclerosis.

Because of their low abundance and structural variability, the quantification of endogenous endocannabinoid is challenging. LC–MS/MS platform enable simultaneous relative and absolute quantification of multiple metabolites in this pathway. The results are widely used for investigating the relative changes in metabolite concentrations, and potential biomarker discovery. Creative Proteomics has established sensitive, reliable, and accurate HPLC-MS/MS method for quantification of endocannabinoid metabolites.

Feature and Advantage of Endocannabinoids Analysis Service

Cutting-edge facilities
Reliable & Reproducible

Sample Requirement

Normal Volume: 200uL plasma; 100mg tissue; 2e7 cells
Minimal Volume: 50uL plasma; 50mg tissue; 5e6 cells

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Ordering Procedure

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Lipidomics Sample Submission Guidelines

Download our Lipidomics Sample Preparation Guide for essential instructions on proper sample collection, storage, and transport for optimal experimental results. The guide covers various sample types, including tissues, serum, urine, and cells, along with quantity requirements for untargeted and targeted lipidomics.

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Metabolomics Sample Submission Guidelines

* For Research Use Only. Not for use in diagnostic procedures.

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From Our Clients

"I recently used their proteomics service for a project analyzing protein interactions in yeast models. The team was very responsive and helped clarify the methodology they employed, which made me feel confident in the results. The data quality was solid, with clear identification of several key proteins involved in our study. Their thorough analysis enabled me to pinpoint specific interactions that I hadn't considered before, which significantly improved the direction of my research. I appreciate their professionalism and support throughout the process."

Sarah Thompson, University of California, Berkeley

"Our lab collaborated with them on a project studying cancer biomarkers. The proteomics analysis provided was detailed and focused, specifically highlighting the differential expression of proteins between healthy and tumor samples. Their clear explanations of the data helped my team understand the biological implications. I also appreciated their willingness to revise the reports based on our feedback, ensuring that we had everything we needed for our publication. This collaborative spirit was invaluable."

Emily Rodriguez, Stanford University

"Our lab worked with them on a project studying the effects of diet on gut microbiota using proteomics. They used a label-free quantification method to analyze proteins in fecal samples before and after dietary intervention. The results showed significant changes in protein expression linked to microbial activity. This was pivotal for our hypothesis about diet-microbiota interactions. The clarity of their data presentation made it easy for our team to integrate these findings into our ongoing research."

Dr. Lisa Wong, University of Toronto

"My experience with Creative Proteomics during the mass spectrometry analysis was excellent. We sent in human saliva and mouse brain tissue samples, which they expertly analyzed using both LC-MS and GC-MS techniques. The results were invaluable, revealing key metabolites in the saliva and identifying biomarkers linked to brain function in the brain tissue."

Dr. Emily Carter, Senior Research Scientist

"The overall service from Creative Proteomics was outstanding. They made the entire process seamless and efficient, allowing us to focus on our research. We worked with leaf and root samples from various Arabidopsis genotypes for targeted metabolomics analysis. Their thorough profiling of primary and secondary metabolites gave us important insights into how the plants respond metabolically to environmental stress."

Dr. Laura Henderson, Plant Physiologist

"We had a pleasant collaboration with Creative Proteomics on mass spectrometry analysis of lipids. They conducted a detailed analysis of lipid species, providing us with important insights into lipid metabolism and its relationship with metabolic syndrome disease states."

Dr. Sarah Mitchell, Research Scientist

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