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Animal Tissue Handling for Proteomics and Metabolomics Services

In the realm of proteomic and metabolomic services, the meticulous handling of animal tissue samples is paramount. At Creative Proteomics, we recognize the unique demands of these services and have designed this in-depth guide to address them.

Step 1: Sample Selection

1.1 Species and Strain

Specifics: Select the animal species and strain that align with the requirements of your protein and metabolomics studies. Recognize the potential genetic variations within strains.
Tools: Access to specialized databases, collaborations with research partners and suppliers.
Considerations: Obtain ethical approvals and the necessary permits for animal studies, especially if specific strains are involved.

1.2 Age and Sex

Specifics: Determine the age and sex of the animals in accordance with the objectives of your protein and metabolomics research.
Tools: Detailed animal records, consultations with experienced personnel.
Considerations: Adhere to ethical guidelines and consider the need for sex-specific studies and ethical treatment of animals.

1.3 Health Status

Specifics: Choose animals with the desired health status, whether they are healthy, diseased, or treated. The health status of animals significantly impacts the characteristics of their tissues.
Tools: Veterinary records, health assessments.
Considerations: Emphasize ethical treatment and documentation, ensuring that animal welfare remains a top priority.

1.4 Sample Size

Specifics: Calculate the required sample size to ensure statistical power and support the experimental design in protein and metabolomics studies.
Tools: Statistical software and sample size calculators.
Considerations: Balance the need for an adequate sample size with ethical and economical considerations.

Step 2: Tissue Collection Techniques

2.1 Surgical Biopsy

Specifics: Suitable for collecting larger tissue samples from specific anatomical locations for protein and metabolomics services.
Tools: Surgical instruments, anesthesia equipment.
Procedure: Perform aseptic surgery, excise the tissue with precision, and handle it carefully to minimize contamination and trauma.
Considerations: Minimize distress to the animals, ensure appropriate postoperative care, and monitor their well-being.

2.2 Needle Biopsy

Specifics: Ideal for collecting small tissue samples with minimal invasiveness, specifically in the context of protein and metabolomics studies.
Tools: Fine needles, syringes, local anesthesia.
Procedure: Gently insert the needle into the tissue, extract the sample, and handle it with care to minimize tissue damage and animal discomfort.
Considerations: Prioritize minimizing tissue trauma and distress to the animals.

2.3 Post-Mortem Collection (Necropsy)

Specifics: Appropriate for pathological studies or post-mortem examination for protein and metabolomics services.
Tools: Autopsy tools, dissection instruments.
Procedure: Conduct a systematic collection of organs and tissues following humane animal euthanasia, ensuring careful documentation.
Considerations: Ethical treatment of animals and accurate documentation are of utmost importance.

Step 3: Preservation of Animal Tissue Samples

3.1 Cryopreservation

Specifics: Suitable for long-term storage and molecular studies in the context of protein and metabolomics services.
Tools: Cryogenic containers, liquid nitrogen, sample labeling equipment.
Procedure: Rapidly freeze tissues in liquid nitrogen, store them in cryovials, and maintain them at ultra-low temperatures.
Considerations: Prevent contamination during freezing and storage, employ meticulous labeling, and ensure a consistent supply of liquid nitrogen.

3.2 Formalin Fixation

Specifics: Ideal for histological analysis in protein and metabolomics services.
Tools: Formalin solution (10%), sealed containers.
Procedure: Immerse tissue samples in formalin to ensure complete coverage, paying attention to fixation time and accurate labeling.
Considerations: Handle samples safely due to formalin's toxicity.

3.3 Snap-Freezing

Specifics: Preserves cellular structures for molecular analyses, which are essential for protein and metabolomics studies.
Tools: Liquid nitrogen, dry ice, cryogenic containers, accurate sample labeling.
Procedure: Quickly immerse tissues in liquid nitrogen or dry ice, then store them in appropriately labeled cryovials.
Considerations: Prevent ice crystal formation, maintain precise labeling, and ensure secure storage.

3.4 RNAlater Solution

Specifics: Safeguards RNA from degradation, particularly valuable in the context of protein and metabolomics research.
Tools: RNAlater solution, sealed containers.
Procedure: Immerse tissues in RNAlater to stabilize RNA, ensuring complete coverage.
Considerations: Store samples at the recommended temperature, use secure containers, and maintain accurate labeling.

* For Research Use Only. Not for use in diagnostic procedures.

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From Our Clients

"I recently used their proteomics service for a project analyzing protein interactions in yeast models. The team was very responsive and helped clarify the methodology they employed, which made me feel confident in the results. The data quality was solid, with clear identification of several key proteins involved in our study. Their thorough analysis enabled me to pinpoint specific interactions that I hadn't considered before, which significantly improved the direction of my research. I appreciate their professionalism and support throughout the process."

Sarah Thompson, University of California, Berkeley

"Our lab collaborated with them on a project studying cancer biomarkers. The proteomics analysis provided was detailed and focused, specifically highlighting the differential expression of proteins between healthy and tumor samples. Their clear explanations of the data helped my team understand the biological implications. I also appreciated their willingness to revise the reports based on our feedback, ensuring that we had everything we needed for our publication. This collaborative spirit was invaluable."

Emily Rodriguez, Stanford University

"Our lab worked with them on a project studying the effects of diet on gut microbiota using proteomics. They used a label-free quantification method to analyze proteins in fecal samples before and after dietary intervention. The results showed significant changes in protein expression linked to microbial activity. This was pivotal for our hypothesis about diet-microbiota interactions. The clarity of their data presentation made it easy for our team to integrate these findings into our ongoing research."

Dr. Lisa Wong, University of Toronto

"My experience with Creative Proteomics during the mass spectrometry analysis was excellent. We sent in human saliva and mouse brain tissue samples, which they expertly analyzed using both LC-MS and GC-MS techniques. The results were invaluable, revealing key metabolites in the saliva and identifying biomarkers linked to brain function in the brain tissue."

Dr. Emily Carter, Senior Research Scientist

"The overall service from Creative Proteomics was outstanding. They made the entire process seamless and efficient, allowing us to focus on our research. We worked with leaf and root samples from various Arabidopsis genotypes for targeted metabolomics analysis. Their thorough profiling of primary and secondary metabolites gave us important insights into how the plants respond metabolically to environmental stress."

Dr. Laura Henderson, Plant Physiologist

"We had a pleasant collaboration with Creative Proteomics on mass spectrometry analysis of lipids. They conducted a detailed analysis of lipid species, providing us with important insights into lipid metabolism and its relationship with metabolic syndrome disease states."

Dr. Sarah Mitchell, Research Scientist

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