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Vitamin A Analysis Service

Vitamin A is also known as anti dry eye vitamin, which is one of the necessary nutrients for human beings. Vitamin A belong to a group of unsaturated nutritional organic compounds, which includes retinol, retinal, retinoic acid, and several proVitamin A carotenoids and the most notably is beta-carotene. Vitamin A is discovered in 1816, the physiologist named François Magendie observed that dogs developed corneal ulcers and had a high mortality rate due to the depriving of nutrition. In 1912, Frederick Gowland Hopkins demonstrated that there are unknown accessory factors existing in milk, which is not the carbohydrates, proteins or fats were necessary for growth in rats and Hopkins received a Nobel Prize for this discovery in 1929. The "accessory factors" were termed "fat soluble" in 1918 and later were termed as "Vitamin A" in 1920.

Scientists at Creative Proteomics utilize a highly quantitative method with high-performance liquid chromatography (HPLC) for the determination of Vitamin A levels in various samples, including Tissue and more. High-Performance Liquid Chromatography (HPLC) with UV detection is used for the determination of Vitamin A (325 nm) levels in a lot of biological samples. This Methodology provides accurate, reliable, and reproducible results of Vitamin A measurement, which enables us to analyze of Vitamin A levels in vitro and in vivo.

Vitamin A has multiple functions, for example, it is important for growth and development. It also plays an important role in maintenance of the immune system and good vision. Vitamin A is necessary by the retina of the eye in the form of retinal, which combines with protein opsin to form rhodopsin, which is the light-absorbing molecule and is necessary for both low-light (scotopic vision) and color vision. Vitamin A also has function as retinoic acid, which is an irreversibly oxidized form of retinol and is an important hormone-like growth factor for epithelial and other cells.
The Russian-Polish botanist M. Tswett is generally recognized as the first person to establish the principles of chromatography. In a paper he presented in 1906, Tswett described how he filled a glass tube with chalk powder (CaCO3) and, by allowing an ether solution of chlorophyll to flow through the chalk, separated the chlorophyll into layers of different colors. He called this technique “chromatography”. Fundamentally, chromatography is a technique used to separate the components contained in a sample. High Performance Liquid Chromatography (HPLC) is a method able to separate non-volatile, thermally unstable, and polar components separate or in a mixture. HPLC is a type of chromatography that, because of its wide application range and quantitative accuracy, is regarded as an indispensable analytical technique, particularly in the field of organic chemistry. It is also widely used as a preparation technique for the isolation and purification of target components contained in mixtures.

Vitamin A Analysis Service at Creative Proteomics supports your research in Vitamin A Analysis. HPLC Based Analysis Service Platform enable us at Creative Proteomics offers you a state-of-the-art Analysis Service.

Sample Type
Tissue and more

Method
High-Performance Liquid Chromatography (HPLC) with UV detection is used for the determination of Vitamin A (325 nm) levels in a lot of biological samples. This Methodology provides accurate, reliable, and reproducible results of Vitamin A measurement, which enables us to analyze of Vitamin A levels in vitro and in vivo.

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Metabolomics Sample Submission Guidelines

Download our Metabolomics Sample Preparation Guide for essential instructions on proper sample collection, storage, and transport for optimal experimental results. The guide covers various sample types, including tissues, serum, urine, and cells, along with quantity requirements for untargeted and targeted metabolomics.

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Metabolomics Sample Submission Guidelines

* For Research Use Only. Not for use in diagnostic procedures.

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"I recently used their proteomics service for a project analyzing protein interactions in yeast models. The team was very responsive and helped clarify the methodology they employed, which made me feel confident in the results. The data quality was solid, with clear identification of several key proteins involved in our study. Their thorough analysis enabled me to pinpoint specific interactions that I hadn't considered before, which significantly improved the direction of my research. I appreciate their professionalism and support throughout the process."

Sarah Thompson, University of California, Berkeley

"Our lab collaborated with them on a project studying cancer biomarkers. The proteomics analysis provided was detailed and focused, specifically highlighting the differential expression of proteins between healthy and tumor samples. Their clear explanations of the data helped my team understand the biological implications. I also appreciated their willingness to revise the reports based on our feedback, ensuring that we had everything we needed for our publication. This collaborative spirit was invaluable."

Emily Rodriguez, Stanford University

"Our lab worked with them on a project studying the effects of diet on gut microbiota using proteomics. They used a label-free quantification method to analyze proteins in fecal samples before and after dietary intervention. The results showed significant changes in protein expression linked to microbial activity. This was pivotal for our hypothesis about diet-microbiota interactions. The clarity of their data presentation made it easy for our team to integrate these findings into our ongoing research."

Dr. Lisa Wong, University of Toronto

"My experience with Creative Proteomics during the mass spectrometry analysis was excellent. We sent in human saliva and mouse brain tissue samples, which they expertly analyzed using both LC-MS and GC-MS techniques. The results were invaluable, revealing key metabolites in the saliva and identifying biomarkers linked to brain function in the brain tissue."

Dr. Emily Carter, Senior Research Scientist

"The overall service from Creative Proteomics was outstanding. They made the entire process seamless and efficient, allowing us to focus on our research. We worked with leaf and root samples from various Arabidopsis genotypes for targeted metabolomics analysis. Their thorough profiling of primary and secondary metabolites gave us important insights into how the plants respond metabolically to environmental stress."

Dr. Laura Henderson, Plant Physiologist

"We had a pleasant collaboration with Creative Proteomics on mass spectrometry analysis of lipids. They conducted a detailed analysis of lipid species, providing us with important insights into lipid metabolism and its relationship with metabolic syndrome disease states."

Dr. Sarah Mitchell, Research Scientist

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