Introduction
Peptide purity refers to the amount of correct peptides in a peptide product. The quality and purity of synthetic
peptides are typically checked by analytical reversed-phase high-performance chromatography (RP-HPLC), followed by
mass spectrometry (MS) with ultraviolet (UV) detection at 210-220 nm. The purity of peptides is a key factor
affecting the results of research projects, which has led to the widespread use of peptide purity analysis in
various industries. Notably, common contaminants in peptide products come from chemical synthesis, culture media, or
incorporation from extraction and purification processes, including peptides with deleted sequences, truncated
sequences, incomplete deprotection, and by-products generated during synthesis or final cleavage. To ensure the
success of various peptide experiments, it may be necessary to remove trifluoroacetic acid (TFA), or to assess the
solubility of hydrophobic peptides prior to performing the desired experiment.
Our Services
With our comprehensive peptide analysis platform, Creative Proteomics has developed a highly
sensitive and specialized platform. We can provide professional peptide purity determination services using
analytical reversed-phase HPLC coupled with mass spectrometry. Each peptide is rigorously monitored during the
analytical cycle according to stringent analytical quality assurance and biosynthetic quality control standards.
Throughout the service cycle, our team of experts begins by determining what level of purity you require, or rather,
which impurities can be tolerated in your application. The most common impurities include:
- Delete sequence.
- Truncated sequences.
- Incompletely deprotected sequences.
- Sequences modified during cleavage (reattachment of protecting groups elsewhere in the peptide).
- Other side reaction products formed during synthesis (e.g. asparagine formation, oxidation products).
- TFA (Trifluoroacetic Acid).
- Acetic acid.
Here, our expert team provides the following peptide purity analysis services.
- Reverse-Phase High Performance Liquid Chromatography
We use advanced reverse-phase HPLC to determine the purity of client peptide samples. In theory, peptide purity is
quantified by the chromatogram produced by reversed phase HPLC, and in addition, we can obtain the number and
relative amount of possible by-products by calculating the peak area.
In addition to RP HPLC, our talented scientists can also provide information on the molecular weight of peptides
determined by mass spectrometry. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry
(MALDI-TOF MS) and electrospray ionization mass spectrometry (ESI MS) are the two most commonly used methods.
*Note: we can provide amino acid analysis (AAA) to determine the amino acid composition in peptide or protein
products, ensuring experimental accuracy and consistency.
What Can We Provide?
- High quality peptide purity assessment.
- Certificate of analysis is included with the analytical data.
- MS and HPLC report files.
Creative Proteomics is a trusted provider of proteomics analysis services. Thanks to our outstanding
scientists and powerful mass spectrometry platform, we off provides global customer with comprehensive and
professional peptide purity analysis service by using RP-HPLC. If you have any questions or specific requirements,
please feel free to contact us. We will fulfill your goal.
