Introduction
Immunoglobulin E (IgE), a class of cytophilic antibodies with a δ chain, is the main antibody involved in the
regulation of the pathogenesis of allergic rhinitis, allergic asthma and eczema. Among the five types of
immunoglobulins, IgE immunoglobulin is the least content in normal human serum with the most unstable to heat, the
shortest half-life, the highest decomposition rate and lowest synthesis rate. It is mainly synthesized by B cells in
the lamina propria lymphoid tissue of the respiratory tract and digestive tract, and is a mediator of allergic
reactions. IgE molecule differs most significantly from that of IgG in respect to the "additional" heavy
chain constant domain and the absence of a hinge region in the ε-chain. The six domains comprising the IgE-Fc, a
dimer of Cε2-Cε3-Cε4 domains, are evolutionarily more ancient than the four-domain IgG-Fc. IgE-Fc resembles the Fc
structure of IgM, the most primitive antibody class, and the Fc domains of avian IgY, the ancestor of IgE and IgG.
Fig. 1. Overall structure and glycosylation.
(Sutton B J, et al., 2019)
Creative Proteomics provides a complete IgE antibody sequencing service. Similar to other antibody
sequencing, our IgE Antibody Sequencing service also is achived by using de novo sequencing methods on the basis of
a powerful mass spectrometry platform. De novo sequencing is our proprietary protein sequencing technology that has
been developed for many years, and we guarantee 100% sequence coverage of the detection results.
Complete IgE de novo sequencing is a very complex process, including multiple different protease hydrolysis, a series
of chemical derivatization reactions, LC-MS data retrieval, bioinformatics analysis and manual mapping analysis with
more than a dozen proprietary software, etc.
At Creative Proteomics, with decades of sequencing experience, our talented scientists have
developed a complete and high quality sequencing process, it roughly includes the following steps:
Ⅰ. Proteolytic digestion and chemical derivatization reactions.
Ⅱ. LC-MS data acquisition.
Ⅲ. Database search and peptide de novo sequencing.
Ⅳ. Preliminary sequence splicing.
Ⅴ. Sequence supplementation.
Ⅵ. Sequence verification.
At Creative Proteomics, our de novo sequencing technology is also applicable to other proteins.
However, some of these steps need to be changed depending on the protein properties.
Sample Requirements
The protein amount is preferably greater than 1mg (purity > 90%) due to the need for multiple different protease
hydrolysis during the sequencing process.
Applications
- Patent application.
- Expression of recombinant monoclonal antibodies.
- Production of monoclonal antibodies.
- Construct chimeric antibodies, humanized antibodies, bispecific antibodies, etc.
Creative Proteomics provides professional IgE antibody sequencing services for global customers. Our
team has successfully sequenced thousands of antibodies to help researchers decode their antibodies in a fraction of
the time. Therefore, please contact us if you are interested in our
antibody sequencing services.
References
- Sutton B J, Davies A M, Bax H J, et al. (2019) IgE Antibodies: From structure to function and clinical
translation. Antibodies (Basel). 8(1):19.
