Antibody De Novo Sequencing
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- Antibody De Novo Sequencing
Service Details
Antibody sequence information is crucial to understanding the structural basis for antigen binding and enables the use of antibodies as therapeutics and research tools. Sequencing of monoclonal antibodies contributes to the development of biopharmaceuticals and diagnostic kits. In particular, primary antibody sequence analysis is the basis for antibody development and the development of antibody drugs for humanization. However, due to the variation and modification of antibody products, the sequence information of most antibodies is often not included in the available databases. De novo sequencing technology uses advanced data processing algorithm for sequence analysis, has a superior advantage versus database dependent traditional methods. Antibody sequencing can be accurately analyzed using the de novo sequencing method, with no need for database searching.
Fig. 1. De novo protein sequencing of monoclonal antibodies. (Peng W W, et al., 2021).
Based on advanced mass spectrometry platform, and combined with rich experience in bioinformatics analysis, Creative Proteomics has established a new generation of antibody de novo sequencing platform to achieve accurate analysis of the primary antibody structure. The method uses a panel of multiple complementary proteases to generate suitable peptides for de novo sequencing by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in a bottom-up fashion. The method achieves full sequence coverage of the monoclonal antibody, with an accuracy of 99% in the variable regions. Our customers have widely used our sequencing methods to achieve desirable results in the field of antibody drug discovery.
Please feel free to contact us If you would like to discuss the best way to determine the sequencing of your antibody. At Creative Proteomics, we provide professional antibody de novo sequencing services for global customers. Our scientists can also design the best solutions according to your specific requirements, and describe them in detailed project reports, we look forward to with your cooperation.
References
For research use only, not intended for any clinical use.