Methylation Protein Analysis Service
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- Methylation Protein Analysis Service
Service Details
Protein methylation is a post-translational modification (PTM) that regulates cellular and biological processes, including transcription, RNA processing, protein interactions, and protein dynamics. Protein methylation occurs on histones and non-histone proteins, usually on arginine and lysine, including monomethylation, symmetric/asymmetric dimethylation and trimethylation, etc.
Traditional protein methylation identification relies primarily on Edman degradation, amino acid analysis, radioisotope labeling, or antibody-based methods (including peptide and protein arrays), but these methods have certain drawbacks, including low throughput, low dynamic range, and nonspecific. In addition, the realization of these methods must rely on prior knowledge of modifications, so it is difficult to accurately identify specific modification sites and differentiate methylation states. In contrast, mass spectrometry (MS)-based techniques are the preferred method for methylation analysis due to their high throughput and wide dynamic range, including bottom-up, mid-bottom and top-down approaches.
Fig. 1. The primary types and mechanisms of protein methylation. (Dai X, et al., 2021)
Creative Proteomics provides a high-throughput, wide dynamic range mass spectrometry platform for quantitative analysis of protein methylation.
At Creative Proteomics, our efficient and professional laboratory technicians selectively enrich methylated peptides by immunoprecipitation using antibodies specific for different methylation sites and modifications prior to MS/MS analysis, which successfully eliminates low stoichiometry caused by transient and unstable methylation modifications, thus improving sensitivity of mass mass spectrometry analysis and enableing large-scale quantitative analysis of methylated proteins.
With cutting-edge equipment and expertise in protein sequencing and mass spectrometry, we provide one-stop methylated protein quantitative analysis services. You only need to tell us the purpose of your experiment and send your samples to us, we will take care of all the follow-up matters of the project.
Our experimental workflow includes:
With our powerful mass spectrometry sequencing platform, Creative Proteomics provides a one-stop shop methylation protein analysis service. Our experienced scientists work with you to develop tailor-made analytical solutions. Please feel free to contact us with any questions regarding methylation protein analysis.
References
For research use only, not intended for any clinical use.