Subcellular Proteomics

Subcellular proteomics is the joint application of subcellular isolation techniques and proteomics, providing a more nuanced view of the dynamic and compartmentalized nature of cellular processes. With rich experience, Creative Proteomics offers high-quality subcellular proteomics services, providing valuable insights into your protein research.

What is Subcellular Proteomics

Subcellular proteomics focuses on the comprehensive analysis of proteins within specific cellular compartments or organelles. Understanding the spatial distribution of proteins is crucial for unraveling the complexity of cellular functions and their regulation. Unlike traditional proteomics that examines the entire cellular proteome, subcellular proteomics provides a more nuanced perspective, shedding light on the dynamic and compartmentalized nature of cellular processes. This approach enables researchers to decipher the intricate networks and functions of proteins within distinct cellular locales.

Fig.1 Schematic of the subcellular structure

The principles of subcellular proteomics involve the isolation and characterization of proteins from specific cellular compartments. Subcellular fractionation techniques, such as differential centrifugation or density gradient centrifugation, are employed to separate organelles and subcellular structures. Once isolated, proteins can be analyzed using various proteomics techniques, including gel electrophoresis, mass spectrometry, and protein profiling. Subcellular proteomics often integrates quantitative methods to determine the relative abundance of proteins within different cellular compartments, providing a deeper understanding of cellular organization and function.

Applications of Subcellular Proteomics

Our Service

With advanced protein detection technology, Creative Proteomics provides targeted solutions for researchers and scientists to help them utilize powerful analytical techniques to reveal the complexity of the proteome. With our thoughtful and detailed services, you can choose any protein analysis, including qualitative, quantitative, targeted and PTM analysis, and we will provide you with a detailed plan according to your requirements. The subcellular proteomics service process is as follows:

Fig.2 Subcellular proteomics workflow

• Isolation of Organelles
  Subcellular proteomics is a joint application of subcellular isolation techniques and proteomics, where isolating and enriching specific organelles is the first step in laying the groundwork. Differential centrifugation, density gradient centrifugation, IP or magnetic beads-based methods are among the options available.
• Protein Extraction
  After organelles isolation, proteins are extracted from the isolated samples. Various methods, including detergent-based lysis or organic solvent extraction, can be employed to obtain a protein-rich fraction
• Detection
  WB, protein microarrays and LC-MS/MS are powerful tools for the identification and quantification of peptides, and they provide detection information that facilitates a comprehensive analysis of organelles proteome.
• Bioinformatics Analysis
  The raw mass spectrometry data undergoes bioinformatics analysis to identify proteins, quantify their abundance, and infer potential functional roles.
• Result Delivery
  After professional data analysis, we deliver results quickly and consistently.

Why Choose Us

• Rich experience in proteomics research
• Excellent research team and professional platform
• Unique and comprehensive research services in the proteomics field
• Reliable data and results

Creative Proteomics brings a wealth of experience to the field of protein research. Our comprehensive services and products cover the entire spectrum of utilizing suitable and efficient gateways. Utilizing our proprietary platform, we have successfully implemented many proteomics research projects. If you are interested, please contact us for more information and a quote.