Exosome Proteomics

Exosome proteomics involves the comprehensive analysis of proteins within exosomes, shedding light on their functional roles and potential applications in various physiological and pathological processes. With rich experience, Creative Proteomics offers high-quality exosome proteomics services, providing valuable insights into your protein research.

What is Exosome Proteomics

Exosome proteomics is a field of study focused on the comprehensive analysis of proteins present in exosomes. Exosomes are small extracellular vesicles that are released by various cell types and play crucial roles in intercellular communication. They carry a cargo of proteins, lipids, nucleic acids, and other biomolecules, reflecting the physiological and pathological states of their originating cells.

Fig.1 Schematic of exosome proteomics (Choi, DS., et al.; 2015)

Exosome proteomics involves the identification, quantification, and characterization of proteins within exosomes. This encompasses understanding the diversity of proteins, their post-translational modifications, and their functional relevance in mediating cellular communication. Advanced mass spectrometry techniques, such as liquid chromatography-mass spectrometry (LC-MS/MS), are central to exosome proteomics. Exosome proteins can be identified and quantified using these techniques at high throughput. Additionally, immunoaffinity-based methods, like immunoprecipitation and Western blotting, are often employed to validate and study specific protein targets within exosomes. The proteins encapsulated in exosomes can provide valuable insights into cellular processes, signaling pathways, and disease mechanisms.

Applications of Exosome proteomics

Exosome proteomics has broad applications in various fields, such as:

Our Service

With advanced protein detection technology, Creative Proteomics provides targeted solutions for researchers and scientists to help them utilize powerful analytical techniques to reveal the complexity of the proteome. With our thoughtful and detailed services, you can choose any protein analysis, including qualitative, quantitative, targeted and PTM analysis, and we will provide you with a detailed plan according to your requirements. The exosome proteomics service process is as follows:

Fig.2 Exosome proteomics workflow

• Isolation of Exosomes
  Exosome proteomics begins with the isolation of exosomes from biological fluids or cell culture supernatants. Common isolation methods include ultracentrifugation, size-exclusion chromatography, and immunoaffinity capture using exosome-specific markers.
• Protein Extraction
  After exosome isolation, proteins are extracted from the exosome samples. Various methods, including detergent-based lysis or organic solvent extraction, can be employed to obtain a protein-rich fraction
• Detection
  WB, protein microarrays and LC-MS/MS are powerful tools for the identification and quantification of peptides, and they provide detection information that facilitates a comprehensive analysis of the exosome proteome.
• Bioinformatics Analysis
  The raw mass spectrometry data undergo bioinformatics analysis to identify proteins, quantify their abundance, and infer potential functional roles. Database searches, pathway analysis, and network analysis are common bioinformatics approaches in exosome proteomics.
• Result Delivery
  After professional data analysis, we deliver results quickly and consistently.

Creative Proteomics brings a wealth of experience to the field of protein research. Our comprehensive services and products cover the entire spectrum of utilizing suitable and efficient gateways. Utilizing our proprietary platform, we have successfully implemented many proteomics research projects. If you are interested, please contact us for more information and a quote.

Reference

• Choi, DS.; et al. Proteomics of extracellular vesicles: Exosomes and ectosomes. Mass Spectrometry Reviews. 2015, 34(4): 474-90