Lactylation Analysis Service

The discovery of lactylation modifications in 2019 has led to new directions for the study of the metabolite lactate's involvement in oncology, immunity, and other fields. Creative Proteomics offers high-quality acylation analysis services, including identification, quantification and characterization, providing valuable insights into your protein research.

What is Lactylation

In 2019, it was found that lactic acid accumulated during metabolism can act as a precursor substance leading to lactate modification of histone lysine and participate in the homeostatic regulation of bacterial-infected M1 macrophages, modulating inflammation, cancer, and other diseases. This broadens the way for the study of lactonization of proteins. Lactonization is one of the important post-translational modifications of proteins and may occur on any protein residue with a free amino group, such as lysine, arginine and tyrosine. In the presence of lactonase (lactoyl-CoA transferase), lactic acid is ester-bonded to the amino acid residues of the substrate protein, lactonizing it.

Fig.1 Schematic of lactylation (Hagihara, H. et al., 2021)

As a type of post-translational modification of proteins, some studies have confirmed that lactylation modification is an important way for lactic acid to exert its function, which is involved in glycolysis-related cellular functions, macrophage polarization, vascular function, mitochondria, nervous system regulation and other important life activities, and can guide new directions for research in the fields of tumors, immunity and so on.

Applications of Lactylation Analysis

• Cellular Signaling and Metabolism
  Explore how lactylation may influence cellular signaling pathways and metabolic processes.
• Disease Research
  Lactylation analysis can reveal the molecular mechanism of lactylation modification in metabolic regulation and immune response, and provide new targets and strategies for the treatment of related diseases.
• Cancer research
  Through lactylation modification histone assay, the biological significance of lactylation modification in tumorigenesis and development can be revealed, providing new indicators and targets for cancer treatment and prognosis assessment.

The study of lactylation has a wide range of applications, including but not limited to the understanding of protein structure and function, the study of disease pathogenesis, and the development of novel drugs, and also contributes to our understanding of the complexity and dynamics of biological systems.

Our Service

Creative Proteomics has advanced protein detection technologies and can provide customized services to researchers and scientists to help them reveal the complexity of PTM using powerful mass spectrometry technologies. Our services are thoughtful and detailed and the process of lactylation analysis service is as follows:

Fig.2 Lactylation analysis workflow

• Sample Preparation
  Proper extraction and preparation of proteins from biological samples to ensure preservation of lactylated proteins.
• Acetylation Enrichment
  Enrichment techniques are employed to isolate lactylated proteins or peptides. This step is crucial as lactylation modifications are often present in low abundance compared to unmodified proteins.
• Detection
  WB, protein microarrays and LC-MS/MS are powerful tools for the identification and quantification of lactylated peptides, and they provide detection information that facilitates a comprehensive analysis of lactylation.
• Bioinformatics Analysis
  Advanced bioinformatics tools are used to analyze data, identify lactylated peptides, and map these modifications to protein sequences.
• Result Delivery
  After professional data analysis, we deliver results quickly and consistently.

Creative Proteomics brings a wealth of experience to the field of protein research. Our comprehensive services and products cover the entire spectrum of utilizing suitable and efficient gateways. Utilizing our proprietary platform, we have successfully implemented many proteomics research projects. If you are interested, please contact us for more information and a quote.

Reference

• Hagihara, H.; et al. Protein lactylation induced by neural excitation. Cell Reports. 2021, 37(2): 109820