Glutarylation Analysis Service
Glutarylation is a newly discovered type of acylation modification in recent years, which is reversible, dynamic, and conserved, and plays essential regulatory roles in genes, metabolism, and the cell cycle. Creative Proteomics offers high-quality glutarylation analysis services, including identification, quantification and characterization of protein glutarylation modifications, providing valuable insights into your protein research.
What is Glutarylation
Protein glutarylation is the enzyme-catalyzed covalent binding of a glutaryl group (e.g., a donor such as glutaryl coenzyme A) to a lysine residue of a substrate protein, and the process is reversible. Lysine itself has a positive charge, and when glutarylation occurs, it changes the positive charge to a negative one, and this change in the protein's charge affects protein conformation and protein-protein interactions.
Fig.1 Schematic of glutarylation (Xie, L. et al.; 2021)
As a newly discovered type of acylation modification in recent years, glutaralylation has significant regulatory effects on nucleosome assembly, chromatin structure, gene expression, DNA damage repair, and cell cycle, and similar to other acylation modifications, it also plays an important role in metabolism, which has been demonstrated to have an effect on nucleosome dynamic structure and sperm motility.
Applications of Glutarylation Analysis
Metabolic Regulation
Explore how glutarylation influences metabolic enzymes and pathways, particularly its role in energy metabolism and cellular homeostasis.
Protein Function and Structure
Examine the effects of glutarylation on protein function and structure, providing insights into its modulatory role in diverse cellular activities.
Immunological research
Glutaric acid is an important regulator of T cell metabolism and differentiation and has a potential role in improving T cell immunotherapy.
Cell Signaling Pathways
Discuss glutarylation's impact on cellular signaling cascades, emphasizing its role in signal transduction and cellular responses.
Our Service
Creative Proteomics has advanced protein detection technologies and can provide customized services to researchers and scientists to help them reveal the complexity of PTM using powerful mass spectrometry technologies. Our services are thoughtful and detailed and the process of glutarylation analysis service is as follows:
Fig.2 Glutarylation analysis workflow
- Sample preparation
Proper extraction and preparation of proteins from biological samples to ensure preservation of glutarylated proteins. - Glutarylation enrichment
Enrichment techniques are employed to isolate glutarylated proteins or peptides. This step is crucial as glutarylation modifications are often present in low abundance compared to unmodified proteins. - Detection
WB, protein microarrays and LC-MS/MS are powerful tools for the identification and quantification of glutarylated peptides, and they provide detection information that facilitates a comprehensive analysis of glutarylation. - Bioinformatics analysis
Advanced bioinformatics tools are used to analyze data, identify glutarylated peptides, and map these modifications to protein sequences. - Result delivery
After professional data analysis, we deliver results quickly and consistently.
Creative Proteomics brings a wealth of experience to the field of protein research. Our comprehensive services and products cover the entire spectrum of utilizing suitable and efficient gateways. Utilizing our proprietary platform, we have successfully implemented many proteomics research projects. If you are interested, please contact us for more information and a quote.
Reference
- Xie, L.; et al. Functions and Mechanisms of Lysine Glutarylation in Eukaryotes. Frontiers in Cell and Developmental Biology. 2021, 9: 667684