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The whole gene expression profile chip was used to analyze the gene expression changes of the customer's existing research target gene A after interference. Combined with the CP disease key gene patent database and the high-content functional gene screening platform (HCS) to screen out those that have not been reported in the studied tumor Downstream gene B, which plays an important regulatory role in tumor cell proliferation. Complete the functional experiments of the B gene at the cellular and animal level, and continue the research project of the target gene A by supplementing the research method.

Preliminary screening of target functional genes: 20 candidate gene shRNA lentiviruses were used to infect target cells, and each gene was replicated in 3 wells to reduce the expression of candidate genes endogenously expressed in target cells. After infection, the cells were counted for 4 to 5 days, and the cell proliferation of the cells after the down-regulation of candidate gene expression was compared with the control cells. About 1-2 genes B that had an effect on cell proliferation were initially selected.

Functional study of functionally positive gene B in vivo: Preparation of tumor cells of target gene knockdown experimental group or control, injection of nude mice, breeding of nude mice until tumor growth, recording animal weight, tumor size curve, and post-mortem Collect tumor masses and process the data results.

Creative Proteomics leads a rich team that uses the most rigorous experimental solutions and internationally recognized experimental techniques for Personalized research plan for new tumor drug targets to obtain your customized information. Use the most accurate experimental results to help you better carry out tumor-related scientific research.