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C-Terminal Sequencing Service

Proteins are macromolecular compounds formed by the dehydration condensation of amino acids, which contain both amino-terminus (-NH2, N-terminus) and carboxy-terminus (-COOH, C-terminus). The C-terminus is an important structural and functional site of proteins. Some specific post-translational modifications, such as C-terminus cleavage, affect the structure of proteins and thus their biological functions. Therefore, studying the amino acid species and sequence of the C-terminal of the protein is helpful to reveal the structure and function of the protein.

With the deepening of proteomics research, C-terminal sequencing plays an increasingly important role in protein function research .Especially for protein biopharmaceuticals, C-end sequencing is essential. Several new protein C-terminal sequencing methods have been established with improved sensitivity and reproducibility that can be applied at the proteome level.

C-terminal sequencing method

There are three methods for protein C-terminal sequencing: carboxypeptidase method, chemical method and tandem mass spectrometry. Currently, the more widely used method is tandem mass spectrometry.

The strategy of carboxypeptidase and chemical methods is to obtain the C-terminal amino acids by enzymatic or chemical reagent cleavage, which is combined with liquid chromatography or mass spectrometry techniques for the identification of amino acid species.

The principle of C-terminal sequence determination by tandem mass spectrometry is to digest the protein sample into peptide mixtures, then analyze the peptide mixtures by tandem mass spectrometry and deduce the complete C-terminal amino acid sequence in combination with corresponding software and database.

C-terminal sequencing service

Creative Proteomics uses tandem mass spectrometry for protein C-terminus sequencing. Our efficient and accurate C-terminus sequencing service is based on advanced ESI and MALDI TOF, which can detect blocked and modified protein C-termini, confirm whether the recombinant protein is fully expressed, detect whether the recombinant protein is broken during expression, and whether the C-terminus sequence of the recombinant protein is modified, etc.

Features

Does not require carboxypeptidase digestion and chemical cleavage;

Short cycle;

High precision and accurate results.

Suitable for all types of C-terminal sequence detection.

Service Flow

C-Terminal Sequencing Service

Sample delivery requirements

The purity of the sample for HPLC detection should not be less than 95%.

The protein amount should be at least 50ug and the protein concentration should not be less than 0.5mg/ml.

The sample should be recombinant protein or known sequence protein.

Proteomics Sample Submission Guidelines

Ensure your samples are prepared and submitted correctly by downloading our comprehensive Proteomics Sample Submission Guidelines. This document provides detailed instructions and essential information to facilitate a smooth submission process. Click the link below to access the PDF and ensure your submission meets all necessary criteria.

Proteomics Sample Submission Guidelines
* For Research Use Only. Not for use in diagnostic procedures.
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