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Succinylation Analysis Service

Protein succinylation is the process by which a succinyl group donor (e.g., succinyl coenzyme A) covalently binds a succinyl group to a lysine residue, either enzymatically or non-enzymatically. Lysine succinylation is widespread in eukaryotic and prokaryotic cells, and many succinylation sites have been identified in many species such as bacteria, fungi, parasites, and mammalian cells, and these modified proteins are found in various parts of cells, including cell membrane, cytoplasmic matrix, various organelles, and nucleus. Lysine succinylations are involved in different metabolic reactions in cells, including glucose metabolism, tricarboxylic acid cycle, and fatty acid metabolism, which are closely related to the activities of living organisms.

Our Succinylation Analysis Service

As an industry leader in PTMs, Creative Proteomics offers a large-scale protein succinylation analytical service package through mass spectrometry and enrichment processing. By using this service, customers will only need to send in the sample of investigation and leave the rest to our team, including but not limited to protein extraction, proteolysis, peptide separation, mass spectrometry analysis, mass spectrometry raw data analysis, and bioinformatics analysis.

Workflow

During our service cycle, your provided protein samples will be digested into a mixture of peptide fragments and then separated by liquid chromatography according to their different components, hence reducing the overall complexity of your protein sample. Subsequently, succinylated peptides are annotated, identified, and specifically enriched using the high-quality succinylated modified antibodies or biological materials for final bioinformatic analysis. As a result, the succinylated protein modification is revealed, analyzed, and quantified by liquid chromatography-tandem mass spectrometry.

Workflow

Technical Advantages

Adopt mainstream antibody affinity enrichment method to achieve high specificity and enrichment efficiency
Large-scale identification of enriched succinylated peptides with mass spectrometry of high resolution and high throughput
Combining commercially available quantitative techniques to analyze, compare, and correlate succinylation levels between different quantitatively
A research team with extensive experience in PTMs

Customized Bioinformatics Services

Standard Analysis	Customized Analysis
a) Collection of output statistics b) Establishment of database and search for modified proteins c) Identification of modified proteins and modification sites d) Annotation of modified proteins e) Quantitative analysis of modified proteins f) Analysis of differential expression of modified proteins g) Functional enrichment analysis of differentially modified proteins GO/KEGG	Modified peptide motif analysis Protein network interaction prediction Protein domain prediction (Advanced Analysis) Protein subcellular localization analysis (Advanced Analysis)

Sample requirements

Fresh animal tissue: ≥600 mg
Fresh plant tissue: ≥6 g
Cell culture: ≥1×10⁷ cells/tube x 3 tubes
Fungi and bacteria: ≥600 mg
Serum, plasma: 450 μL × 4 tubes
Protein solution: total protein of 5-10 mg
Body fluid samples: urine of 15 mL × 4 tubes (centrifuge at 1000 x g for 5 minutes and discard sediment); or other body fluids (saliva, amniotic fluid, cell culture supernatant, etc.) > 15 mL

Applications

Drug target research
Disease biomarker screening
Plant stress/resistance research
mechanism of action research

Technology platforms

Ion Chromatography
High-Performance Liquid Chromatography (HPLC)
Matrix-Assisted Laser Desorption Ionization Mass Spectrometry (MALDI-MS)

Proteomics Sample Submission Guidelines

Ensure your samples are prepared and submitted correctly by downloading our comprehensive Proteomics Sample Submission Guidelines. This document provides detailed instructions and essential information to facilitate a smooth submission process. Click the link below to access the PDF and ensure your submission meets all necessary criteria.

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* For Research Use Only. Not for use in diagnostic procedures.

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From Our Clients

"I recently used their proteomics service for a project analyzing protein interactions in yeast models. The team was very responsive and helped clarify the methodology they employed, which made me feel confident in the results. The data quality was solid, with clear identification of several key proteins involved in our study. Their thorough analysis enabled me to pinpoint specific interactions that I hadn't considered before, which significantly improved the direction of my research. I appreciate their professionalism and support throughout the process."

Sarah Thompson, University of California, Berkeley

"Our lab collaborated with them on a project studying cancer biomarkers. The proteomics analysis provided was detailed and focused, specifically highlighting the differential expression of proteins between healthy and tumor samples. Their clear explanations of the data helped my team understand the biological implications. I also appreciated their willingness to revise the reports based on our feedback, ensuring that we had everything we needed for our publication. This collaborative spirit was invaluable."

Emily Rodriguez, Stanford University

"Our lab worked with them on a project studying the effects of diet on gut microbiota using proteomics. They used a label-free quantification method to analyze proteins in fecal samples before and after dietary intervention. The results showed significant changes in protein expression linked to microbial activity. This was pivotal for our hypothesis about diet-microbiota interactions. The clarity of their data presentation made it easy for our team to integrate these findings into our ongoing research."

Dr. Lisa Wong, University of Toronto

"My experience with Creative Proteomics during the mass spectrometry analysis was excellent. We sent in human saliva and mouse brain tissue samples, which they expertly analyzed using both LC-MS and GC-MS techniques. The results were invaluable, revealing key metabolites in the saliva and identifying biomarkers linked to brain function in the brain tissue."

Dr. Emily Carter, Senior Research Scientist

"The overall service from Creative Proteomics was outstanding. They made the entire process seamless and efficient, allowing us to focus on our research. We worked with leaf and root samples from various Arabidopsis genotypes for targeted metabolomics analysis. Their thorough profiling of primary and secondary metabolites gave us important insights into how the plants respond metabolically to environmental stress."

Dr. Laura Henderson, Plant Physiologist

"We had a pleasant collaboration with Creative Proteomics on mass spectrometry analysis of lipids. They conducted a detailed analysis of lipid species, providing us with important insights into lipid metabolism and its relationship with metabolic syndrome disease states."

Dr. Sarah Mitchell, Research Scientist

Online Inquiry

Please submit a detailed description of your project. We will provide you with a customized project plan to meet your research requests. You can also send emails directly to for inquiries.

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