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RNA Pull Down Service

The interaction between RNA and protein is one of the main mechanisms of protein function, which is the key to many cellular functions, such as protein synthesis, mRNA assembly, virus replication, and regulation of cell development. As an important means to detect the interaction between RNA and its binding protein, RNA pull-down is of indispensable significance.

With the advent of the era of genes, RNA-related research has become an important part of biology. It has been widely studied in the fields of tumors, chronic diseases such as diabetes, and enteritis, but the related mechanisms are still poorly understood. The interaction of various newly discovered IncRNAs, circRNAs, and proteins needs further elucidation. In recent years, RNA pull-down has developed into the major tool to study the interaction of IncRNAs and circRNAs with proteins.

About RNA Pull Down

Initially, the RNA molecule is labeled (such as biotin probe labeling) and then incubated with cell lysate to form the RNA-protein complex. This complex can be bound to streptavidin-labeled magnetic beads, thereby separating itself from other components in the incubation solution. After elution of the complex, proteins can be detected via the western blot for proteins of known identifications or mass spectrometry for proteins of unknown identification.

Our RNA Pull-down Service

Creative Proteomics offers RNA pull-down service using specific secondary antibodies to avoid the interference of heavy chains of antibodies. Creative Proteomics is equipped with the world's most advanced mass spectrometer to enhance the detection results in terms of efficiency and reliability.

Our RNA Pull Down Service

Advantages

Low-abundance enrichment: enrich the detection of low-abundance target protein-RNA complexes

Mature proteomics platform: provides western blot and mass spectrometry identification of RNA pull-down complexes

Professional bioinformatics analysis

Sample Requirements

Sample Type: cell or tissue
Sample size: a) Cells > 5×10⁸ b) Tissue > 500 mg
Sample Shipping: Shipping on dry ice
Sample preservation: cell samples or fresh tissue stored in liquid nitrogen and at -80°C

Highlights

High precision
Provide various complete controls to ensure rigorous results
Provide prediction and identification of important functional regions of proteins

Delivery

Sequencing report
SDS-PAGE silver staining of RNA pull-down protein
Mass spectrometry identification report

Proteomics Sample Submission Guidelines

Ensure your samples are prepared and submitted correctly by downloading our comprehensive Proteomics Sample Submission Guidelines. This document provides detailed instructions and essential information to facilitate a smooth submission process. Click the link below to access the PDF and ensure your submission meets all necessary criteria.

Download

* For Research Use Only. Not for use in diagnostic procedures.

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From Our Clients

"I recently used their proteomics service for a project analyzing protein interactions in yeast models. The team was very responsive and helped clarify the methodology they employed, which made me feel confident in the results. The data quality was solid, with clear identification of several key proteins involved in our study. Their thorough analysis enabled me to pinpoint specific interactions that I hadn't considered before, which significantly improved the direction of my research. I appreciate their professionalism and support throughout the process."

Sarah Thompson, University of California, Berkeley

"Our lab collaborated with them on a project studying cancer biomarkers. The proteomics analysis provided was detailed and focused, specifically highlighting the differential expression of proteins between healthy and tumor samples. Their clear explanations of the data helped my team understand the biological implications. I also appreciated their willingness to revise the reports based on our feedback, ensuring that we had everything we needed for our publication. This collaborative spirit was invaluable."

Emily Rodriguez, Stanford University

"Our lab worked with them on a project studying the effects of diet on gut microbiota using proteomics. They used a label-free quantification method to analyze proteins in fecal samples before and after dietary intervention. The results showed significant changes in protein expression linked to microbial activity. This was pivotal for our hypothesis about diet-microbiota interactions. The clarity of their data presentation made it easy for our team to integrate these findings into our ongoing research."

Dr. Lisa Wong, University of Toronto

"My experience with Creative Proteomics during the mass spectrometry analysis was excellent. We sent in human saliva and mouse brain tissue samples, which they expertly analyzed using both LC-MS and GC-MS techniques. The results were invaluable, revealing key metabolites in the saliva and identifying biomarkers linked to brain function in the brain tissue."

Dr. Emily Carter, Senior Research Scientist

"The overall service from Creative Proteomics was outstanding. They made the entire process seamless and efficient, allowing us to focus on our research. We worked with leaf and root samples from various Arabidopsis genotypes for targeted metabolomics analysis. Their thorough profiling of primary and secondary metabolites gave us important insights into how the plants respond metabolically to environmental stress."

Dr. Laura Henderson, Plant Physiologist

"We had a pleasant collaboration with Creative Proteomics on mass spectrometry analysis of lipids. They conducted a detailed analysis of lipid species, providing us with important insights into lipid metabolism and its relationship with metabolic syndrome disease states."

Dr. Sarah Mitchell, Research Scientist

Online Inquiry

Please submit a detailed description of your project. We will provide you with a customized project plan to meet your research requests. You can also send emails directly to for inquiries.

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