Shikimic Acid is a cyclohexene, a cyclitol and a cyclohexanecarboxylic acid and is more commonly known as shikimate, which is the anionic form of Shikimic Acid. Shikimic Acid is an important biochemical intermediates and products of metabolism in plants and microorganisms. Shikimic Acid was isolated by Johan Fredrik Eykman, a Dutch chemist in 1885 from theapanese flower shikimiand its name is also comes from the Japanese flower. The chemical structure of Shikimic Acid was elucidated by by Hermann O. L. Fischer and German chemist Gerda dangschat until 1934. Shikimic Acid mainly is isolated from the dried ripe fruit of Illiciaceae plants octagonal or Illicium anisatum (Japanese star anise), and there are also have reported that Shikimic Acid can be extracted from the sweetgum fruit and pine needles in recent years.
Scientists at Creative Proteomics utilize a highly quantitative method with high-performance liquid chromatography (HPLC) for the determination of Shikimic Acid levels in various samples, including Plants, Fungi, Microbes and more. High-Performance Liquid Chromatography (HPLC) using a differential refractive index detector (RID) for the determination of Shikimic Acid levels in a lot of biological samples. This Methodology provides accurate, reliable, and reproducible results of Shikimic Acid measurement, which enables us to analyze of Shikimic Acid levels in vitro and in vivo.
Shikimic Acid is a biosynthetic intermediate and found in very low concentrations in generally although it is present in most autotrophic organisms. There are some chemical research papers reporting that Shikimic Acid can be extracted in yields of around 1.5% from the seeds of the sweetgum (Liquidambar styraciflua) fruit, which is abundant in North America. Biosynthetic pathways in E. coli have recently been improved to allow the organism to accumulate enough material to be used commercially.
The Russian-Polish botanist M. Tswett is generally recognized as the first person to establish the principles of chromatography. In a paper he presented in 1906, Tswett described how he filled a glass tube with chalk powder (CaCO3) and, by allowing an ether solution of chlorophyll to flow through the chalk, separated the chlorophyll into layers of different colors. He called this technique “chromatography”. Fundamentally, chromatography is a technique used to separate the components contained in a sample. High Performance Liquid Chromatography (HPLC) is a method able to separate non-volatile, thermally unstable, and polar components separate or in a mixture. HPLC is a type of chromatography that, because of its wide application range and quantitative accuracy, is regarded as an indispensable analytical technique, particularly in the field of organic chemistry. It is also widely used as a preparation technique for the isolation and purification of target components contained in mixtures.
Shikimic Acid Analysis Service at Creative Proteomics supports your research in Shikimic Acid Analysis. HPLC Based Analysis Service Platform enable us at Creative Proteomics offers you a state-of-the-art Analysis Service.
Sample Type
Plants, Fungi, Microbes and more
Method
High-Performance Liquid Chromatography (HPLC) using a differential refractive index detector (RID) for the determination of Shikimic Acid levels in a lot of biological samples. This Methodology provides accurate, reliable, and reproducible results of Shikimic Acid measurement, which enables us to analyze of Shikimic Acid levels in vitro and in vivo.
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