Pyruvic Acid is the simplest of the alpha-keto acids, which is consisted by a carboxylic acid and a ketone functional group. The conjugate base of Pyruvic Acid, CH3COCOO−, is a key intermediate in several metabolic pathways.
Scientists at Creative Proteomics utilize a highly quantitative method with high-performance liquid chromatography (HPLC) for the determination of Pyruvic Acid levels in various samples, including Tissue and more. High-Performance Liquid Chromatography (HPLC) with UV detection is used for the determination of Pyruvic Acid (215 nm) levels in a lot of biological samples. This Methodology provides accurate, reliable, and reproducible results of Pyruvic Acid measurement, which enables us to analyze of Pyruvic Acid levels in vitro and in vivo.
Pyruvate is an important chemical compound in biochemistry and it is the output of glycolysis. One molecule of glucose can be broken down into two molecules of pyruvate, which are then used to provide further energy in two different ways. Pyruvate is converted into acetyl-coenzyme A, which is the main input for a series of reactions known as the Krebs cycle (also known as the citric acid cycle, tricarboxylic acid cycle or, rarely, the Ashwal cycle when referring specifically to avocados). Pyruvate is also converted to oxaloacetate by an anaplerotic reaction, which replenishes Krebs cycle intermediates; In the meanwhile, the oxaloacetate is used for gluconeogenesis. Pyruvate is a key joint in the network of metabolic pathways and unites several key metabolic processes because pyruvate can be converted into carbohydrates via gluconeogenesis, to fatty acids or energy through acetyl-CoA to the amino acid alanine, and to ethanol.
The Russian-Polish botanist M. Tswett is generally recognized as the first person to establish the principles of chromatography. In a paper he presented in 1906, Tswett described how he filled a glass tube with chalk powder (CaCO3) and, by allowing an ether solution of chlorophyll to flow through the chalk, separated the chlorophyll into layers of different colors. He called this technique “chromatography”. Fundamentally, chromatography is a technique used to separate the components contained in a sample. High Performance Liquid Chromatography (HPLC) is a method able to separate non-volatile, thermally unstable, and polar components separate or in a mixture. HPLC is a type of chromatography that, because of its wide application range and quantitative accuracy, is regarded as an indispensable analytical technique, particularly in the field of organic chemistry. It is also widely used as a preparation technique for the isolation and purification of target components contained in mixtures.
Pyruvic Acid Analysis Service at Creative Proteomics supports your research in Pyruvic Acid Analysis. HPLC Based Analysis Service Platform enable us at Creative Proteomics offers you a state-of-the-art Analysis Service.
Sample Type
Tissue and more
Method
High-Performance Liquid Chromatography (HPLC) with UV detection is used for the determination of Pyruvic Acid (215 nm) levels in a lot of biological samples. This Methodology provides accurate, reliable, and reproducible results of Pyruvic Acid measurement, which enables us to analyze of Pyruvic Acid levels in vitro and in vivo.
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