Corticosterone, is also known as 17-deoxycortisol and 11β,21-dihydroprogesterone and the molecular formula of Corticosterone is C21H30O4. Corticosterone is a a 21-carbon steroid hormone, which is produced in the cortex of the adrenal glands in rodents and other non-human animals.
Scientists at Creative Proteomics utilize a highly quantitative method with high-performance liquid chromatography (HPLC) for the determination of Corticosterone levels in various samples, including Serum, Tissue and more. High-Performance Liquid Chromatography (HPLC) with UV detection is used for the determination of Corticosterone (240 nm) levels in a lot of biological samples. This Methodology provides accurate, reliable, and reproducible results of Corticosterone measurement, which enables us to analyze of Corticosterone levels in vitro and in vivo.
Corticosterone is a main glucocorticoid, which is a class of steroid hormones in many species, including amphibians, reptiles, rodents and birds and is involved in regulation of energy, immune reactions, and stress responses. However, Corticosterone has very weak glucocorticoid and mineralocorticoid activities in humans and just as mainly as an intermediate in the steroidogenic pathway from pregnenolone to aldosterone because cortisol is the most important glucocorticoid which is produced primarily in the zona fasciculata of the adrenal cortex. Aldosterone synthase, which is is a steroid hydroxylase cytochrome P450 enzyme involved in the biosynthesis of the mineralocorticoid aldosterone, can converte Corticosterone to aldosterone and the biochemical process is only found in the mitochondria of glomerulosa cells. Corticosterone is also the precursor substrate for the biosynthesis of the mineralocorticoid aldosterone, which is one of the dominant homeostatic modulators to regulate the levels of sodium and potassium in vivo. Corticosterone also plays multiple roles in regulation of the process of memory and the main effects is on the modulating the stress on the emotional memories and the impact of long term memory.
The Russian-Polish botanist M. Tswett is generally recognized as the first person to establish the principles of chromatography. In a paper he presented in 1906, Tswett described how he filled a glass tube with chalk powder (CaCO3) and, by allowing an ether solution of chlorophyll to flow through the chalk, separated the chlorophyll into layers of different colors. He called this technique “chromatography”. Fundamentally, chromatography is a technique used to separate the components contained in a sample. High Performance Liquid Chromatography (HPLC) is a method able to separate non-volatile, thermally unstable, and polar components separate or in a mixture. HPLC is a type of chromatography that, because of its wide application range and quantitative accuracy, is regarded as an indispensable analytical technique, particularly in the field of organic chemistry. It is also widely used as a preparation technique for the isolation and purification of target components contained in mixtures.
Corticosterone Analysis Service at Creative Proteomics supports your research in Corticosterone Analysis. HPLC Based Analysis Service Platform enable us at Creative Proteomics offers you a state-of-the-art Analysis Service.
Sample Type
Serum, Tissue and more
Method
High-Performance Liquid Chromatography (HPLC) with UV detection is used for the determination of Corticosterone (240 nm) levels in a lot of biological samples. This Methodology provides accurate, reliable, and reproducible results of Corticosterone measurement, which enables us to analyze of Corticosterone levels in vitro and in vivo.
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