Dandelion, belonging to compositae, is very common in the mountains, paths, riverside and even in our yard. Dandelion has very light smell and it tastes a little bitter.
What’s more, it has the ability to treat some diseases, such as, swollen boils poison, mastitis, red eyes, sore throat and many other symptoms that we cannot ignore.
Dandelion contains many kinds of pharmaceutical ingredients and nutrients. Especially the C9H8O4 has a good drug efficacy. This post will introduce the process of C9H8O4 detection in dandelion with high performance liquid chromatography.
Theory
Weighing some dandelion sample and putting it into methanol solution with 5% formic acid to be dissolved. And then it can be injected into high performance liquid chromatography system after some process, such as, centrifugation, filtration and ultrasonic extraction. In this system, we can get the content of C9H8O4 in dandelion with the help of separation of C18 columns, UV detection equipment and external marking algorithm.
Equipment and reagents
Equipment: high performance liquid chromatography, ultrasonic cleaning, solvent filters, electronic scales, centrifuge.
Reagents: methanol, formic acid, phosphoric acid, ultrapure water and sodium dihydrogen phosphate.
Sample preparation
1. Preparation of the reference solution
Weighing 2 mg C9H8O4 and putting into 50 ml volumentric flask. And this sample will be dissolved by methanol and then we will get 40 ug/ml C9H8O4 reference solution.
2. Preparation of the test solution
Preparing proper dandelion and putting 1 g meal into 50 ml erlenmeyer flask with cork. And then adding 10 ml methanol solution with 5% formic acid to the erlenmeyer flask . After the process of shaking, weighing and sonication, it will be took out and weighed. Using the methanol solution with 5% formic acid to fill the vacancy. Filtering the solution with organic phase micro-membrane and storing it in a brown bottle.
3. Chromatography conditions
A. Detector: UV detector
B. Detection wavelength: 323 nm
C. Columns: promosil C18 columns
D. Mobile phase: methanol : Phosphate buffer = 25 : 75
E. Velocity of flow: 10 ml/min
F. Column temperature: 40 Celsius
G. The injection volume: 10 ul
Conclusion
According to many kinds of authentication methods, this method---High performance liquid chromatography has good standard for data detection. Its advantages lie in THE short time, good resolution, concise operation. It is suitable to detect the C9H8O4 in dandelion.
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